短链脂肪酸丁酸增强巨噬细胞预防问号钩端螺旋体的急性感染  

Role and mechanism of butyric acid in hamster leptospirosis

作　　者：陈曦 谢旭峰 刘鑫 刘久茜 张文龙 曹永国 CHEN Xi;XIE Xufeng;LIU Xin;LIU Jiuxi;ZHANG Wenlong;CAO Yongguo(College of Veterinary Medicine,Jilin University,Changchun 130062,China)

机构地区：[1]吉林大学动物医学学院,吉林长春130062

出　　处：《中国兽医学报》2024年第8期1743-1748,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(32172872);中国博士后科学基金资助项目(BX20220131)。

摘　　要：钩端螺旋体病(钩体病)是一种全球性自然疫源性人兽共患传染病。研究表明,肠道菌群代谢产物短链脂肪酸参与宿主免疫调节、影响疾病进程。本试验旨在探究肠道菌群代谢产物短链脂肪酸在钩体病中的作用。结果表明,补充短链脂肪酸丁酸能显著提高金黄地鼠钩体病存活率。体外试验表明,丁酸处理抑制感染钩体后巨噬细胞Cat和Gsr基因表达,但上调了NOX1和NOX4基因表达。同时,丁酸刺激增加了感染钩体巨噬细胞ROS水平,而高水平的ROS可增强巨噬细胞杀菌功能。体内试验也证实丁酸通过调节ROS的产生保护金黄地鼠抵抗急性钩端螺旋体病。综上所述,肠道菌群代谢产物短链脂肪酸丁酸通过调节ROS表达增强巨噬细胞杀菌功能,从而保护宿主抵抗钩体病。Leptospirosis is a global natural zoonotic infectious disease.Research shows that short-chain fatty acids,metabolites of gut microbiota,are involved in host immune regulation and affect disease progression.This experiment was conducted to investigate the role of short-chain fatty acids in leptospirosis.The results showed that supplementation of short-chain fatty acid butyric acid could significantly improve the survival rate of leptospirosis in hamsters.In vitro experiments showed that butyric acid treatment inhibited the expression of Cat and Gsr genes in macrophages after infection with Leptospira,but enhanced the expression of NOX1 and NOX4 genes.At the same time,butyric acid treatment enhanced macrophage ROS levels after leptospirosis infection,and high levels of ROS enhanced the bactericidal function of macrophages.In vivo experiments also confirmed that butyric acid protects hamsters against acute leptospirosis by regulating ROS production.Collectively,the short-chain fatty acid butyric acid,a metabolite of gut microbiota,enhances the bactericidal function of macrophages by regulating ROS expression,thereby protecting the host against leptospirosis.

关 键 词：丁酸 钩体病 ROS 巨噬细胞 肠道菌群 

分 类 号：R535[医药卫生—内科学]