Permeable and robust polymer-silica hybrid armor on cell catalyst for sustainable biomanufacturing  

作　　者：Yiran Cheng Zhenhua Wu Boyu Zhang Jiaxu Zhang Jiafu Shi Zhongyi Jiang 

机构地区：[1]School of Environmental Science and Engineering,Tianjin University,Tianjin,300072,China [2]Key Laboratory for Green Chemical Technology of Ministry of Education,School of Chemical Engineering and Technology,Tianjin University,Tianjin,300072,China

出　　处：《Particuology》2024年第9期106-112,共7页颗粒学报（英文版）

基　　金：the National Key Research and Development Program of China(grant No.2021YFC2102300);National Natural Science Foundation of China(grant No.22122809);Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(grant No.TSBICIP-KJGG-003);Open Funding Project of the State Key Laboratory of Biochemical Engineering,China(grant No.2020 KF-06);Haihe Laboratory of Sustainable Chemical Transformations,and Program of Introducing Talents of Discipline to。

摘　　要：Inactivated cell catalysis is one of several central techniques in green biomanufacturing realm.However,the instability and leakage of enzymes in inactivated cell severely restrict the practical applications of inactivated cell catalysis.Constructing armor on the surface of inactivated cells affords a feasible and effective strategy to enhance the stability of cells while commonly lowering the permeability.Herein,polymer-silica hybrid armor(PSHA)is directly generated on the surface of enzyme-containing cells.The branched structure of PEI enables higher porosity of cell@PSHA,exhibiting 1.52-fold enhancement in substrate permeability by contrast with cell@silica armor(SA).The electrostatic interactions(NH_(3)^(+)with O^(−))and hydrogen bonding(N⋯H or O⋯H)interactions between structural units enables higher stability of cell@PSHA,showing 3.13-fold elevation in Young's modulus compared with [e-mail] a result,the cell@PSHA can catalyze continuous conversion of starch to tagatose for 15 batches over 969 h,with an average yield of 77.76 g L^(−1).

关 键 词：Biomanufacturing Wholecell catalysis Polymer-silica hybrid armor Tagatose production Starch conversion 

分 类 号：O643.36[理学—物理化学]