Investigating the genetic diversity of several varieties of Iranian tomato (Solanum lycopersicum) using RAPD and ISSR molecular markers  

作　　者：Bahareh Ahang Sanaz Pashapour Aida Ghasemi Abbas Zabihi 

机构地区：[1]Department of Biology,Faculty of Basic Sciences,Islamic Azad University,RashtBranch,Rasht 0098813,Iran [2]Department of Pharmacology and toxicology,Faculty of Pharmacy and Pharmaceutical Sciences,Islamic Azad University,Medical Sciences Branch,Tehran,Iran [3]School of Medicine,Medical University of Hamedan,Hamedan,Iran

出　　处：《Food and Health》2024年第4期19-26,共8页食品和健康（英文）

摘　　要：Background:Numerous studies have demonstrated the existence of approximately 7,500 genetic tomato varieties worldwide.Hence,it is crucial to assess the genetic diversity among tomato cultivars.This study aimed to investigate the genetic diversity of selected Iranian tomato cultivars(Solanum lycopersicum)using RAPD and ISSR molecular markers.Method:Ten RAPD primers and ten ISSR primers were employed to assess the genetic diversity among 10 tomato cultivars:Matin,RFT 112,Hirad,Golsar,Raha,Hengam,Hedah,Fasa,JS12,and Emerald.Data analysis involved the UPGMA algorithm and NTYSYSpc software.Results:RAPD analysis revealed close genetic proximity between Fasa and JS12,as well as between Raha and Hadieh.Conversely,the RFT 112,Hengam,Hirad,and Emerald cultivars exhibited significant genetic diversity within this group.ISSR primer analysis identified Hengam as the most diverse variety,while Matin,Emerald,and Vibrid,as well as Raha and JS12,displayed genetic similarities with minimal observed diversity.Furthermore,the overall analysis of the cultivars using RAPD and ISSR markers indicated that Hengam exhibited the highest diversity among all the varieties.Notably,Raha and JS12 demonstrated limited diversity in this analysis.Conclusion:This research demonstrates substantial genetic diversity among the investigated tomato varieties,with Hengam displaying the highest diversity within this group.Furthermore,ISSR markers proved more effective in determining genetic diversity in tomato plants.

关 键 词：Solanum lycopersicum TOMATO genetic diversity RAPD ISSR 

分 类 号：S64[农业科学—蔬菜学]