基于Smart-seq2探究玻璃化冷冻对猪孤雌激活囊胚基因表达的影响  

Exploring the Effect of Vitrification on Gene Expression in Porcine Parthenogenetic Blastocysts by Smart-seq2

作　　者：杨柏高 龙熙[1] 张亮[1] 徐皆欢 戴建军[3] 赵学明[2] 潘红梅[1] YANG Baigao;LONG Xi;ZHANG Liang;XU Jiehuan;DAI Jianjun;ZHAO Xueming;PAN Hongmei(Chongqing Academy of Animal Sciences,Chongqing 402460,China;Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Institute of Animal Science and Veterinary Science,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China)

机构地区：[1]重庆市畜牧科学院,重庆402460 [2]中国农业科学院北京畜牧兽医研究所,北京100193 [3]上海市农业科学院畜牧兽医研究所,上海201106

出　　处：《畜牧兽医学报》2024年第9期3936-3946,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：重庆市技术创新与应用发展专项重点项目(cstc2021jscx-dxwtBX0004);重庆地方猪胚胎冷冻保存技术研究与猪育种新材料创制(2022-CHQ-01-01);重庆市人民政府与中国农业科学院战略合作资助项目(23310)

摘　　要：旨在探究玻璃化冷冻对猪孤雌激活(parthenogenetic activation,PA)囊胚基因表达的影响。本研究以猪PA囊胚为研究对象,根据试验处理分为新鲜组、玻璃化冷冻组Ⅰ和玻璃化冷冻组Ⅱ,随后从每组挑选3枚形态良好的囊胚,利用Smart-seq2单细胞全长转录组测序技术进行转录组测序分析。结果显示,玻璃化冷冻组Ⅰ与新鲜组相比,共鉴定到772个差异表达基因(differential expression genes,DEGs),GO和KEGG富集分析结果显示,这些DEGs与细胞脂质代谢过程、细胞葡萄糖稳态、MAPK信号通路、PI3K-Akt信号通路等相关;玻璃化冷冻组Ⅱ与新鲜组相比,共鉴定到1613个DEGs,主要与糖异生、代谢途径、氨基酸生物合成等相关;玻璃化冷冻组Ⅱ与玻璃化冷冻组Ⅰ相比,鉴定到822个DEGs,主要与丙酮酸代谢过程、N-聚糖生物合成、细胞衰老等相关。综上,本研究基于Smart-seq2单细胞全长转录组测序技术揭示了玻璃化冷冻对猪PA囊胚脂质代谢、能量代谢、MAPK信号通路等相关基因表达的影响。The aim of this study was to investigate the effect of vitrification on gene expression in porcine parthenogenetic activation(PA)blastocysts.In this study,porcine PA blastocysts were divided into fresh,vitrification group I and vitrification group II according to the experimental treatments,and then 3 well-morphologised blastocysts were selected from each group and analysed by transcriptome sequencing using Smart-seq2 single-cell full-length transcriptome sequencing technology.As a result,compared with the fresh group,a total of 772 differential expression genes(DEGs)were identified in the vitrification groupⅠ.GO and KEGG enrichment analysis results showed that these DEGs were related to cellular lipid metabolic process,cell glucose homeostasis,MAPK signaling pathway,PI3K-Akt signaling pathway,etc.Compared with the fresh group,a total of 1613 DEGs were identified in the vitrification groupⅡ,which mainly related to gluconeogenesis,metabolic pathways,biosynthesis of amino acids,etc.Compared with the vitrification groupⅠ,822 DEGs were identified in the vitrification groupⅡ,which mainly involved in pyruvate metabolic process,N-Glycan biosynthesis,cellular senescence,etc.In summary,this study revealed the effects of vitrification on the expression of genes related to lipid metabolism,energy metabolism,MAPK signalling pathway and other related genes in porcine PA blastocysts based on Smart-seq2 single-cell full-length transcriptome sequencing technology.

关 键 词：Smart-seq2 玻璃化冷冻 猪 孤雌激活 囊胚 

分 类 号：S828.3[农业科学—畜牧学]