陈皮精油对脂多糖诱导的RAW264.7细胞炎症的干预作用  

Intervention of Essential Oils from Citrus reticulata Blanco on Lipopolysaccharide-induced Inflammation in RAW264.7 Cells

作　　者：刘玲 史万玉[1] 李秀梅[2] 王明华 翟向和[1] 周炜炜 LIU Ling;SHI Wanyu;LI Xiumei;WANG Minghua;ZHAI Xianghe;ZHOU Weiwei(College of Chinese Veterinary Medicine,Hebei Agricultural University,Baoding 071000,China;Key Laboratory of Feed Biotechnology,Ministry of Agriculture and Rural Affairs,Institute of Feed Research,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]河北农业大学中兽医学院,保定071000 [2]中国农业科学院饲料研究所农业农村部饲料生物技术重点实验室,北京100081

出　　处：《畜牧兽医学报》2024年第9期4153-4160,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：农业农村部饲料生物技术重点实验室开放课题(KLFB-FRI-202104);中国农科院创新工程联合攻关重大科研任务(CAAS-ZDRW202111)。

摘　　要：基于脂多糖(lipopolysaccharide,LPS)诱导的RAW264.7细胞模型研究陈皮精油抗炎作用。通过Griess法检测一氧化氮(nitric oxide,NO)分泌水平,采用Real-time PCR法检测细胞中肿瘤坏死因子(tumor necrosis factorα,TNF-α)、白细胞介素6(interleukin-6,IL-6)、白细胞介素1β(interleukin-1β,IL-1β)和诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)、环氧化酶2(cyclooxygenase 2,COX-2)mRNA的表达水平。结果显示,陈皮精油显著降低LPS诱导的RAW264.7细胞释放NO的含量,抑制iNOS、COX-2、IL-1β、IL-6和TNF-αmRNA的表达。综上所述,陈皮精油通过抑制炎症因子的分泌和表达,有效缓解由LPS诱导RAW264.7细胞炎症反应。The anti-inflammatory effect of essential oils from Citrus reticulata Blanco was investigated based on lipopolysaccharide(LPS)-induced RAW264.7 cell model.The level of Nitric oxide(NO)secretion was detected by Griess method,and the mRNA expression levels of tumor necrosis factor(TNF-α),interleukin-6(IL-6),interleukin-1β(IL-1β),inducible nitric oxide synthase(iNOS)and cyclooxygenase 2(COX-2)were determined by real-time PCR method.The results showed that essential oils from Citrus reticulata Blanco significantly decreased the level of NO released from LPS-induced RAW264.7 cells and inhibited the expression of iNOS,COX-2,IL-1β,IL-6 and TNF-αmRNA.In conclusion,essential oils from Citrus reticulata Blanco can inhibit the secretion and expression of inflammatory factors,and thus alleviate the inflammatory response induced by LPS in RAW264.7 cells effectively.

关 键 词：陈皮 精油 抗炎 LPS RAW264.7细胞 

分 类 号：S853.7[农业科学—临床兽医学]