表达绿色荧光蛋白的重组伪狂犬病病毒构建及在中和试验中应用  

Construction of recombinant pseudorabies virus expressing green fluorescent protein and its application in neutralization test

作　　者：任晓祥 刘立辉 肖娜 张帅[1] 武月 赵云环 刘涛[3] 左玉柱[1] 范京惠[1] REN Xiaoxiang;LIU Lihui;XIAO Na;ZHANG Shuai;WU Yue;ZHAO yunhuan;LIU Tao;ZUO Yuzhu;FAN Jinghui(College of Veterinary Medicine,Hebei Agricultural University,Baoding 071001,China;Dingzhou Bureau of Agricultural and Rural Affairs,Dingzhou 073000,China;Ringpu(Baoding)Biological Pharmaceutical Co.,Ltd.,Baoding 071001,China)

机构地区：[1]河北农业大学动物医学院,河北保定071001 [2]定州市农业农村局,河北定州073000 [3]瑞普(保定)生物药业有限公司,河北保定071001

出　　处：《中国兽医科学》2024年第9期1248-1252,共5页Chinese Veterinary Science

基　　金：河北省农业产业技术体系生猪创新团队项目(HBCT2024220201);河北省农业产业技术体系生猪创新团队项目(HBCT2024220401)。

摘　　要：为了优化病毒血清中和抗体试验方法,减少传统病毒中和抗体试验的周期,本研究通过同源重组技术,构建出能够表达绿色荧光蛋白的伪狂犬g E基因缺失病毒PRV-Δg E-AcGFP1。将其作为检测病毒,对g E抗体阴性血清进行病毒血清中和抗体试验,测定中和抗体效价。结果显示,利用重组病毒测定中和抗体效价的结果与传统方法无显著差异。结果表明,PRV-Δg E-AcGFP1可作为攻击病毒用于血清中和抗体试验,为进行方便、准确、周期短的血清中和抗体试验提供一种新的选择。In order to optimize the viral serum neutralizing antibody test method and reduce the cycle time of traditional viral neutralizing antibody test,a pseudorabies gE gene deletion virus PRV-ΔgE-AcGFP1 expressing green fluorescent protein was constructed by homologous recombination technology,and used it as a test virus for determination of neutralizing antibody potency by performing the viral serum neutralizing antibody test on gE antibody-negative sera.The results showed that there was no significant difference between the recombinant virus and the traditional method in determining the titer of neutralizing antibody.The results show that PRV-ΔgE-AcGFP1 could be used as test virus for serum neutralizing antibody assay,providing a new option for performing convenient,accurate and short-cycle serum neutralizing antibody tests.

关 键 词：伪狂犬病病毒 同源重组 绿色荧光蛋白 中和试验 

分 类 号：S852.659.1[农业科学—基础兽医学]