依托咪酯通过下调circ_0000527表达对人视网膜母细胞瘤Y-79细胞生物学行为的影响  

Effects of etomidate on the biological behavior of human retinoblastoma Y-79 cells by down-regulating the expression of circ_0000527

作　　者：王立功[1] 张继青 WANG Li-gong;ZHANG Ji-qing(Department of Anesthesia,The Second People's Hospital of Lanzhou,Lanzhou 730046,Gansu Province,China)

机构地区：[1]兰州市第二人民医院麻醉科,甘肃兰州730046

出　　处：《中国临床药理学杂志》2024年第18期2699-2703,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的探讨依托咪酯(ETO)对人视网膜母细胞瘤Y79细胞的生物学行为影响以及机制研究。方法体外培养Y-79细胞,分为对照组(常规培养)、低、中、高剂量实验组(用0.2、0.4、0.8μg·mL^(-1)ETO处理)、si-NC组(转染si-NC)、si-circ_0000527组(转染si-circ_0000527)、ETO+pcDNA组(用0.8μg·mL^(-1)ETO处理后转染pcDNA)、ETO+pcDNA-circ_0000527组(用0.8μg·mL^(-1)ETO处理后转染pcDNA-circ_0000527)。用MTT法检测细胞增殖情况;用平板克隆实验检测细胞克隆形成情况;用划痕实验和Transwell小室检测细胞迁移及侵袭能力;用实时荧光定量聚合酶链反应(qRT-PCR)法检测circ_0000527表达情况;用蛋白质印迹法检测N-cadherin、E-cadherin蛋白表达情况。结果对照组、低、中、高剂量实验组、si-NC组、si-circ_0000527组、ETO+pcDNA组、ETO+pcDNA-circ_0000527组的细胞增殖抑制率分别为0、(17.89±1.69)%、(38.43±3.10)%、(56.97±5.06)%、(5.78±0.56)%、(48.79±4.65)%、(58.40±4.81)%和(21.23±2.17)%;克隆形成数量分别为(87.15±6.11)、(70.91±6.26)、(52.32±5.19)、(38.68±3.42)、(88.73±6.81)、(44.18±4.83)、(36.26±3.62)和(72.14±5.69)个;划痕愈合率分别为(71.02±5.58)%、(54.08±4.97)%、(39.67±3.26)%、(23.98±2.77)%、(72.01±5.61)%、(30.16±2.26)%、(22.82±2.15)%和(60.17±5.83)%;侵袭细胞个数分别为(119.80±12.93)、(88.78±6.78)、(70.73±6.18)、(52.78±4.29)、(120.18±12.48)、(62.34±5.88)、(50.88±4.07)和(92.06±8.60)个;circ_0000527表达水平分别为1.00±0.00、0.79±0.06、0.57±0.04、0.35±0.03、1.00±0.00、0.28±0.03、1.00±0.00和3.66±0.23。低、中、高剂量实验组上述指标与对照组比较,si-circ_0000527组上述指标与si-NC组比较,ETO+pcDNA-circ_0000527组上述指标与ETO+pcDNA组比较,在统计学上差异均有统计学意义(均P<0.05)。结论ETO可能通过抑制circ_0000527表达,阻滞Y-79细胞的迁移、侵袭及增殖。Objective To investigate the effect of etomidate(ETO)on the biological behavior of human retinoblastoma Y-79 cells and its possible mechanism.Methods Y-79 cells were cultured in vitro.They were divided into control group(conventional culture),experimental-L,-M,-H groups(treated with 0.2,0.4 and0.8μg·mL^(-1)ETO),si-NC group(transfected with si-NC),sicirc0000527 group(transfected with si-circ0000527),ETO+pcDNA group(transfected pcDNA after treated with0.8μg·mL^(-1)ETO)and ETO+pcDNA-circ0000527 group(transfected pcDNA-circ0000527 after treatment with 0.8μg·mL^(-1)ETO).Cell proliferation was detected by methyl thiazolyl tetrazolium assay;cell clone formation was detected by plate cloning assay;scratch test and Transwell chamber were used to detect he ability of cell migration and invasion;the expression of circ0000527 was detected by real-time fluorescence quantitative polymerase chain reaction;Western blot was used to detect the expression of N-cadherin and E-cadherin.Results The inhibition rates of cell proliferation in control group,experimental-L,-M,-H groups,si-NC group,si-circ0000527group,ETO+pcDNA group and ETO+pcDNA-circ0000527 group were 0,(17.89±1.69)%,(38.43±3.10)%,(56.97±5.06)%,(5.78±0.56)%,(48.79±4.65)%,(58.40±4.81)%and(21.23±2.17)%;the number of clones formed were 87.15±6.11,70.91±6.26,52.32±5.19,38.68±3.42,88.73±6.81,44.18±4.83,36.26±3.62 and 72.14±5.69;scratch healing rates were(71.02±5.58)%,(54.08±4.97)%,(39.67±3.26)%,(23.98±2.77)%,(72.01±5.61)%,(30.16±2.26)%,(22.82±2.15)%and(60.17±5.83)%;the number of invasive cells were 119.80±12.93,88.78±6.78,70.73±6.18,52.78±4.29,120.18±12.48,62.34±5.88,50.88±4.07 and 92,06±860;the expression levels of circ0000527 were 1.00±0.00,0.79±0.06,0.57±0.04,0.35±0.03,1.00±0.00,0.28±0.03,1.00±0.00 and 3.66±0.23,respectively.There were statistically significant differences in the above indexes between experimental-L,-M,-H groups and control group,between sicirc0000527 group and si-NC group,between ETO+pcDNA-circ0000527 group and

关 键 词：依托咪酯 circ_0000527 人视网膜母细胞瘤 细胞增殖 细胞迁移 细胞侵袭 

分 类 号：R97[医药卫生—药品]