子痫前期孕妇胎盘组织自噬相关蛋白的表达及其临床意义  

The expression and clinical significance of autophagy-related proteins in the placental tissues of pregnant women with preeclampsia

作　　者：顾梦薇 丁丽娟 陈晨 Gu Meng-wei;Ding Li-juan;Chen Chen(Department of Obstetrics,The Affiliated Wuxi People's Hospital of Nanjing Medical University(Wuxi Medical Center,Nanjing Medical University),Wuxi,Jiangsu 214002,China)

机构地区：[1]南京医科大学附属无锡人民医院(南京医科大学无锡医学中心)产科,江苏无锡214002

出　　处：《中国现代医学杂志》2024年第18期77-82,共6页China Journal of Modern Medicine

基　　金：江苏省自然科学基金(No:BK20210496)。

摘　　要：目的探讨子痫前期孕妇胎盘组织自噬相关蛋白LC3-Ⅱ、LC3-Ⅰ和LC3B的表达及其临床意义。方法选取2021年8月—2023年8月在南京医科大学附属无锡人民医院产科就诊的子痫前期孕妇108例,其中早发型子痫前期孕妇54例(早发组),晚发型子痫前期孕妇54例(晚发组);另取同期在该院就诊的健康孕妇30例作为对照组。采用逆转录聚合酶链反应和Western blotting检测各组孕妇胎盘组织LC3B mRNA、LC3B和LC3-Ⅱ/LC3-Ⅰ蛋白表达。结果早发组孕妇胎盘组织LC3B mRNA和蛋白相对表达量、LC3-Ⅱ/LC3-Ⅰ均高于对照组和晚发组(P<0.05)。晚发组与对照组孕妇胎盘组织LC3B mRNA和蛋白相对表达量、LC3-Ⅱ/LC3-Ⅰ比较,差异均无统计学意义(P>0.05)。早发组≥30岁孕妇胎盘组织LC3B mRNA和蛋白相对表达量、LC3-Ⅱ/LC3-Ⅰ均高于<30岁孕妇(P<0.05)。Spearman相关性分析显示,早发组孕妇年龄与LC3B mRNA、LC3B蛋白、LC3-Ⅱ/LC3-Ⅰ均呈正相关(rs=0.292、0.299和0.279,均P<0.05)。早发组不同BMI、孕妇类型胎盘组织LC3B mRNA和蛋白相对表达量、LC3-Ⅱ/LC3-Ⅰ比较,差异均无统计学意义(P>0.05)。晚发组不同年龄、BMI、孕妇类型胎盘组织LC3B mRNA和蛋白相对表达量、LC3-Ⅱ/LC3-Ⅰ比较,差异均无统计学意义(P>0.05)。结论胎盘组织中自噬相关蛋白的表达在子痫前期中具有特定的模式,尤其在早发型子痫前期孕妇中更为显著,并且这种表达与孕妇年龄相关。Objective To explore the expression and clinical significance of autophagy-related proteins(LC3-Ⅱ,LC3-Ⅰ,and LC3B)in the placental tissues of pregnant women with preeclampsia.Methods A total of 108 pregnant women with preeclampsia who visited the Department of Obstetrics of our hospital from August 2021 to August 2023 were selected for this study,which included 54 pregnant women with early-onset preeclampsia(earlyonset group)and 54 with late-onset preeclampsia(late-onset group).Additionally,30 healthy pregnant women who visited the hospital during the same period were taken as a control group.The mRNA expression of LC3B and protein expressions of LC3B,and LC3-Ⅱ/LC3-Ⅰin the placental tissues of pregnant women in each group were measured by quantitative real-time polymerase chain reaction and Western blotting,respectively.Results In the early-onset group,the relative mRNA and protein expressions of LC3B,as well as the LC3-Ⅱ/LC3-Ⅰratio in placental tissues of pregnant women,were significantly higher than those in the late-onset and control groups(P<0.05).There was no significant difference in the relative mRNA and protein expressions of LC3B and the LC3-Ⅱ/LC3-Ⅰratio between the late-onset group and the control group(P>0.05).In the early-onset group,pregnant women aged≥30 years had higher relative mRNA and protein expressions of LC3B and LC3-Ⅱ/LC3-Ⅰratio compared with those aged<30 years(P<0.05).The Spearman correlation analysis revealed that there were positive correlations between the age of pregnant women and the mRNA and protein expressions of LC3B and the LC3-Ⅱ/LC3-Ⅰratio in the early-onset group(rs=0.292,0.299,and 0.279,all P<0.05).No significant difference in the relative mRNA and protein expressions of LC3B and the LC3-Ⅱ/LC3-Ⅰratio was observed among those with different BMI and types of pregnancy in the early-onset group(P>0.05),or among those with different ages,BMI and types of pregnancy in the late-onset group(P>0.05).Conclusions The expressions of autophagy-related proteins i

关 键 词：妊娠期高血压疾病 子痫前期 胎盘组织 自噬相关蛋白 

分 类 号：R714.246[医药卫生—妇产科学]