低温对乌龟稚龟应激水平和天然免疫能力的影响  

Effects of low temperature on stress level and innate immunity in juvenile Mauremys reevesii

作　　者：张志强[1] 江文卿 伍亮 毕嘉璐 张青 陈义肯 ZHANG Zhiqiang;JIANG Wenqing;WU Liang;BI Jialu;ZHANG Qing;CHEN Yiken(Engineering Technology Research Center of Healthy Aquaculture,School of Animal Science and Technology,Anhui Agricultural University,Hefei 230036)

机构地区：[1]安徽农业大学动物科技学院,水产动物健康养殖工程技术研究中心,合肥230036

出　　处：《安徽农业大学学报》2024年第4期610-614,共5页Journal of Anhui Agricultural University

基　　金：安徽省舒城县“三区”科技人才项目(2022);安徽农业大学2021年度省级线下课程项目(2021xxkc039);校级质量工程培育项目一流教材《动物学实验指导》项目(2021auyljc01);动物学教学创新团队项目(2023aujxtd004);安徽农业大学2023年省级大学生创新创业训练计划项目创新训练项目(S202210364034)共同资助。

摘　　要：为探究乌龟(Mauremys reevesii)稚龟对低温胁迫的生理适应性,采用背甲下静脉窦采血技术,在6、17和26℃条件下分别处理第1、10和28天时,测定了其身体大小、应激水平和血浆杀菌能力的变化。结果显示:(1)温度和处理时间均不影响稚龟的体重、背甲长和背甲宽(P> 0.05)。(2)6、17和26℃组嗜异性粒细胞与淋巴细胞的比值均不随处理时间而变化(P> 0.05),只在第1天时,6℃组显著低于17℃组(P <0.05)。(3)随处理时间延长,血浆杀菌能力17℃组显著下降(P <0.05),26℃组显著升高(P <0.05);只在第28天时,6℃组和17℃组均显著低于26℃组(P <0.05)。低温对乌龟稚龟的生长无明显的抑制作用,短期低温胁迫导致其应激反应能力增强,天然免疫能力明显下降,不利于其适应入眠前和出眠后环境温度的大幅度波动。In order to explore the physiological adaptation to low temperature threatens in juvenile Chinese three-keeled pond turtle,Mauremys reevesii,the variations of body size,stress level,and plasma bactericidal capacity were measured on day 1,day 10 and day 28,respectively,under the conditions of 6,17 and 26℃by using of dorsal cervical sinus blood sampling technique.The results were as follows.(1)Both different temperatures and treatment time had no great effects on the body mass,the length and width of carapace in juvenile turtles(P>0.05).(2)The ratio of heterophils to lymphocytes did not change with treatment time at any temperature group(P>0.05),only on day 1,it was significantly lower at 6℃than that at 17℃(P<0.05).(3)With prolonged treatment time,plasma bactericidal capacity was greatly declined at 17℃group(P<0.05),but it was significantly increased at 26℃group(P<0.05),only on day 28,it was significantly lower at the groups of 6℃and 17℃than that of 26℃group(P<0.05).Lower temperatures have no marked inhibitory effect on the growth of juvenile turtles,short-term cold temperature threatens produce stronger stress response,accompanying with significantly reduced innate immunity,which are not beneficial for the juvenile turtles to adapt to the large-scale fluctuation of environmental temperature before hibernation and post-hibernation.

关 键 词：乌龟 低温 血浆杀菌能力 应激 

分 类 号：S917.4[农业科学—水产科学]