厨余垃圾废液中解钾菌的筛选及液体菌肥制备  

Screening of Potassium Solubilizing Bacteria in Kitchen Waste Liquid and Preparation of Liquid Microbial Fertilizer

作　　者：许涵秋 胡冰冰 詹艺舒 林标声[2,3] XU Hanqiu;HU Bingbing;ZHAN Yishu;LIN Biaosheng(Longyan Institute of Quality Inspection for Products,Longyan,Fujian 364000,China;Longyan University,Longyan,Fujian 364000,China)

机构地区：[1]龙岩市产品质量检验所,福建龙岩364000 [2]龙岩学院,福建龙岩364000 [3]预防兽医学与生物技术福建省高等学校重点实验室,福建龙岩364000

出　　处：《龙岩学院学报》2024年第5期69-74,87,共7页Journal of Longyan University

基　　金：福建省市场监督管理局科技项目(FJMS2022049)。

摘　　要：从厨余垃圾废液中筛选得到的一株高效解钾菌株LYBM04,解钾效率为65.67%,经16S rDNA确定其为胶质芽孢杆菌(Bacillus mucilaginosus)。LYBM04菌株在LB培养基和解钾菌复筛培养基具有相似的生长曲线,但在解钾菌复筛培养基中整体速度稍慢。通过正交试验,确定了LYBM04菌株可与解磷菌、固氮菌以2∶2∶1(V/V)比例、接种量5%的最优条件下,接入到厨余废液中,30℃好氧发酵40 h制备出液体微生物菌肥,其总磷、氨氮、总氮、总钾等营养成分经过检测,比厨余垃圾废液含量更高,对盆栽玉米的株高、根长、根重均有显著的促生作用,在液态微生物菌肥的开发与应用方面具有一定的应用前景。This study screened a highly efficient potassium solubilizing bacterial strain LYBM04 from kitchen waste liquid,with a potassium solubilizing efficiency of 65.67%.After 16S rDNA analysis,it was identified as Bacillus mucilaginosus.The LYBM04 strain showed similar growth curves in the LB medium and potassium solubilizing bacteria re-screening medium,but the overall speed was slightly slower in potassium solubilizing bacteria re-screening medium.Through orthogonal experiments,it was determined that the LYBM04 strain can be inoculated into kitchen waste liquid with phosphate solubilizing bacteria and nitrogen fixing bacteria in the optimal ratio of 2∶2∶1 and an inoculation amount of 5%for aerobic fermentation at 30℃for 40 hours to prepare liquid microbial fertilizer.After testing,the total phosphorus,ammonia nitrogen,total nitrogen,total potassium and other nutritional components were detected to be higher than those in kitchen waste liquid.It had a significant promoting effect on the plant height,root length,and root weight of potted corn,and had certain application prospects in the development and application of liquid microbial fertilizer.

关 键 词：厨余垃圾 解钾菌 胶质芽孢杆菌 液体菌肥 

分 类 号：Q93-31[生物学—微生物学]