Genetic dissection and origin of pleiotropic loci underlying multilevel fiber quality traits in upland cotton(Gossypium hirsutum L.)  

作　　者：Hongge Li Shurong Tang Zhen Peng Guoyong Fu Yinhua Jia Shoujun Wei Baojun Chen Muhammad Shahid Iqbal Shoupu He Xiongming Du 

机构地区：[1]Zhengzhou Research Base,State Key Laboratory of Cotton Bio-breeding and Integrated Utilization/School of Agricultural Sciences,Zhengzhou University,Zhengzhou 450001,China [2]State Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Institute of Cotton Research,Chinese Academy of Agricultural Sciences,Anyang 455000,China

出　　处：《Journal of Integrative Agriculture》2024年第10期3250-3263,共14页农业科学学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2022YFD1200300);the Central Plain Scholar Program,China(234000510004);the National Supercomputing Center in Zhengzhou,China。

摘　　要：Cotton fiber quality is a persistent concern that determines planting benefits and the quality of finished textile products.However,the limitations of measurement instruments have hindered the accurate evaluation of some important fiber characteristics such as fiber maturity,fineness,and neps,which in turn has impeded the genetic improvement and industrial utilization of cotton fiber.Here,12 single fiber quality traits were measured using Advanced Fiber Information System(AFIS)equipment among 383 accessions of upland cotton(Gossypium hirsutum L.).In addition,eight conventional fiber quality traits were assessed by the High Volume Instrument(HVI)System.Genome-wide association study(GWAS),linkage disequilibrium(LD)block genotyping and functional identification were conducted sequentially to uncover the associated elite loci and candidate genes of fiber quality traits.As a result,the previously reported pleiotropic locus FL_D11 regulating fiber length-related traits was identified in this study.More importantly,three novel pleiotropic loci(FM_A03,FF_A05,and FN_A07)regulating fiber maturity,fineness and neps,respectively,were detected based on AFIS traits.Numerous highly promising candidate genes were screened out by integrating RNA-seq and qRT-PCR analyses,including the reported GhKRP6 for fiber length,the newly identified GhMAP8 for maturity and GhDFR for fineness.The origin and evolutionary analysis of pleiotropic loci indicated that the selection pressure on FL_D11,FM_A03 and FF_A05 increased as the breeding period approached the present and the origins of FM_A03 and FF_A05 were traced back to cotton landraces.These findings reveal the genetic basis underlying fiber quality and provide insight into the genetic improvement and textile utilization of fiber in G.hirsutum.

关 键 词：upland cotton single fiber quality pleiotropic loci candidate genes locus origin 

分 类 号：S562[农业科学—作物学]