Cotton ethylene response factor Gh ERF91 is involved in the defense against Verticillium dahliae  

作　　者：Nurimanguli Aini Yuanlong Wu Zhenyuan Pan Yizan Ma Qiushuang An Guangling Shui Panxia Shao Dingyi Yang Hairong Lin Binghui Tang Xin Wei Chunyuan You Longfu Zhu Dawei Zhang Zhongxu Lin Xinhui Nie 

机构地区：[1]Key Laboratory of Oasis Eco-agriculture,Xinjiang Production and Construction Group/Agricultural College,Shihezi University,Shihezi 832003,China [2]National Key Laboratory of Crop Genetic Improvement/College of Plant Sciences&Technology,Huazhong AgriculturalUniversity,Wuhan 430070,China [3]Cotton Research Institute,Shihezi Academy of Agricultural Science,Shihezi 832011,China [4]Institute of Economic Crops,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China

出　　处：《Journal of Integrative Agriculture》2024年第10期3328-3342,共15页农业科学学报（英文版）

基　　金：supported by the fund for National Key Research and Development Program of China(2023YFD2301203-05);the BTNYGG,China(NYHXGG,2023AA102);the Key Programs for Science and Technology Development of Shihezi City,Xinjiang Production and Construction Corps,China(2022NY01)。

摘　　要：Verticillium dahliae causes significant losses in cotton production.To reveal the mechanism of the defense response to V.dahliae in cotton,transcriptomic analyses were performed using cotton cultivars M138(V.dahliae-resistant)and P2(V.dahliae-susceptible).The results revealed 11,076 and 6,640 differentially expressed genes(DEGs)in response to V.dahliae,respectively.The weighted gene co-expression network analysis of 4,633 transcription factors(TFs)indicated a“MEblue”module containing 654 TFs that strongly correlate with resistance to V.dahliae.Among these TFs,the ethylene response factor Ghi_A05G10166(GhERF91)was identified as a putative hub gene with a defense response against V.dahliae.A virus-induced gene silencing assay and exogenous application of ethephon showed that GhERF91 is activated by ethylene and positively regulates the response to V.dahliae exposure in cotton.This study provides fundamental transcriptome data and a putative causal gene(GhERF91)associated with resistance to V.dahliae,as well as genetic resources for breeding V.dahliae-resistant cotton.

关 键 词：COTTON Verticillium wilt RNA-sequencing GhERF91 virus-induced gene silencing 

分 类 号：S435.621.2[农业科学—农业昆虫与害虫防治]