Optimizing the Bacillus thuringiensis(Bt)protein concentration in cotton:Coordinated application of exogenous amino acids and EDTA to reduce spatiotemporal variability in boll and leaf toxins  

作　　者：Zhenyu Liu Shu Dong Yuting Liu Hanjia Li Fuqin Zhou Junfeng Ding Zixu Zhao Yinglong Chen Xiang Zhang Yuan Chen Dehua Chen 

机构地区：[1]Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of GrainCrops,Yangzhou University,Yangzhou 225009,China [2]The UWA Institute of Agriculture,School of Agriculture and Environment,The University of Western Australia,Perth 6009,WA,Australia

出　　处：《Journal of Integrative Agriculture》2024年第10期3419-3436,共18页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31901462 and 31671613);the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(22KJA210005);the China Scholarship Council(202308320440);the Postgraduate Research&Practice Innovation Program of Jiangsu Province,China(KYCX22_3508)。

摘　　要：During the boll formation stage,cotton bolls exhibit the lowest expression of Bacillus thuringiensis(Bt)insecticidal proteins.Resistance to insects varies notably among different organs,which poses challenges for controlling cotton bollworms.Consequently,an experimental strategy was designed in the 2020-2021 cotton growing season to coordinate the enhancement of protein synthesis and the attenuation of degradation.Two Bt cultivars of Gossypium hirsutum,namely the hybrid Sikang 3 and the conventional Sikang 1,were used as test materials.Three treatments were applied at the peak flowering period:CK(the control),T1(amino acids),and T2(amino acids and EDTA).The results show that,in comparison to the CK group,the Bt protein contents were significantly increased in both cotton bolls and their subtending leaves under the T1 and T2 treatments.The maximum levels of increase observed were 67.5%in cotton bolls and 21.7%in leaves.Moreover,the disparity in Bt protein content between cotton bolls and their subtending leaves notably decreased by 31.2%.Correlation analysis suggested that the primary physiological mechanisms for augmenting Bt protein content involve increased protein synthesis and reduced protein catabolism,which are independent of Bt gene expression levels.Stepwise regression and path analysis revealed that elevating the soluble protein content and transaminase activity,while reducing the catabolic enzyme activities,are instrumental in enhancing the Bt protein content.Consequently,the coordinated application of amino acids and EDTA emerges as a strategy that can improve the overall resistance of Bt cotton and mitigate the spatiotemporal variations in Bt toxin concentrations in both cotton bolls and leaves.

关 键 词：Gossypium hirsutum Bt cotton insecticidal protein bolls and their subtending leaves nitrogen metabolism 

分 类 号：S435.62[农业科学—农业昆虫与害虫防治]