Identification of candidate genes for early-maturity traits by combining BSA-seq and QTL mapping in upland cotton(Gossypium hirsutum L.)  

作　　者：Liang Ma Tingli Hu Meng Kang Xiaokang Fu Pengyun Chen Fei Wei Hongliang Jian Xiaoyan Lü Meng Zhang Yonglin Yang 

机构地区：[1]State Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Institute of Cotton Research,Chinese Academy ofAgricultural Sciences,Anyang 455000,China [2]School of Agriculture,Henan Institute of Science and Technology,Xinxiang 453003,China [3]Shihezi Academy of Agricultural Sciences,Shihezi 832099,China

出　　处：《Journal of Integrative Agriculture》2024年第10期3472-3486,共15页农业科学学报（英文版）

基　　金：funded by the Natural Science Foundation of Xinjiang Uygur Autonomous Region,China(2022D01B222);the China Agriculture Research System(CARS-15-06);the Key R&D Project of Eight Division of Xinjiang Production and Construction Corps,China(2021NY01)。

摘　　要：Cotton breeding for the development of early-maturing varieties is an effective way to improve multiple cropping indexes and alleviate the conflict between grains and cotton in the cultivated fields in China.In the present study,we aimed to identify upland cotton quantitative trait loci(QTLs)and candidate genes related to early-maturity traits,including whole growth period(WGP),flowering timing(FT),node of the first fruiting branch(NFFB),height of the node of the first fruiting branch(HNFFB),and plant height(PH).An early-maturing variety,CCRI50,and a latematuring variety,Guoxinmian 11,were crossed to obtain biparental populations.These populations were used to map QTLs for the early-maturity traits for two years(2020 and 2021).With BSA-seq analysis based on the data of population 2020,the candidate regions related to early maturity were found to be located on chromosome D03.We then developed 22 polymorphic insertions or deletions(InDel)markers to further narrow down the candidate regions,resulting in the detection of five and four QTLs in the 2020 and 2021 populations,respectively.According to the results of QTL mapping,two candidate regions(InDel_G286-InDel_G144 and InDel_G24-InDel_G43)were detected.In these regions,three genes(GH_D03G0451,GH_D03G0649,and GH_D03G1180)have nonsynonymous mutations in their exons and one gene(GH_D03G0450)has SNP variations in the upstream sequence between CCRI50 and Guoxinmian 11.These four genes also showed dominant expression in the floral organs.The expression levels of GH_D03G0451,GH_D03G0649 and GH_D03G1180 were significantly higher in CCRI50 than in Guoxinmian 11 during the bud differentiation stages,while GH_D03G0450 showed the opposite trend.Further functional verification of GH_D03G0451 indicated that the GH_D03G0451-silenced plants showed a delay in the flowering time.The results suggest that these are the candidate genes for cotton early maturity,and they may be used for breeding early-maturity cotton varieties.

关 键 词：cotton genomics early-maturity traits BSA-seq QTL mapping molecular breeding 

分 类 号：S562[农业科学—作物学]