The Clausena lansium genome provides new insights into alkaloid diversity and the evolution of the methyltransferase family  

作　　者：Yongzan Wei Yi Wang Fuchu Hu Wei Wang Changbin Wei Bingqiang Xu Liqin Liu Huayang Li Can Wang Hongna Zhang Zhenchang Liang Jianghui Xie 

机构地区：[1]State Key Laboratory of Biological Breeding of Tropical Crops,Institute of Tropical Bioscience and Biotechnology,ChineseAcademy of Tropical Agricultural Sciences,Haikou 571101,China [2]Key Laboratory of Genetic Resources Evaluation and Utilization of Tropical Fruits and Vegetables(Co-construction by Ministryand Province),Ministry of Agriculture and Rural Affairs/Institute of Tropical Fruit Trees,Hainan Academy of Agricultural Sciences,Haikou 571100,China [3]Beijing Key Laboratory of Grape Sciences and Enology,CAS Key Laboratory of Plant Resources,Institute of Botany,ChineseAcademy of Sciences,Beijing 100093,China [4]Key Laboratory of Tropical Fruit Biology(Ministry of Agriculture and Rural Affairs),South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang 524091,China [5]Haikou Experimental Station,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China [6]Sanya Institute of Breeding and Multiplication/School of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,China [7]Sanya Research Academy,Chinese Academy of Tropical Agricultural Sciences,Sanya 572019,China

出　　处：《Journal of Integrative Agriculture》2024年第10期3537-3553,共17页农业科学学报（英文版）

基　　金：supported by the Central Public-interest Scientific Institution Basal Research Fund for the Chinese Academy of Tropical Agricultural Sciences(1630062019010 and 1630062020010);the Fund of Protection of Species Resources for the Ministry of Agriculture and Rural Affairs of China(125A0605)。

摘　　要：Wampee(Clausena lansium)is an important evergreen fruit tree native to southern China that has a long history of use for medicinal purposes.Here,a chromosome-level genome of C.lansium was constructed with a genome size of 282.9 Mb and scaffold N50 of 30.75 Mb.The assembled genome contains 48.70%repetitive elements and 24,381 protein-coding genes.Comparative genomic analysis showed that C.lansium diverged from Aurantioideae 15.91-24.95 million years ago.Additionally,some expansive and specific gene families related to methyltransferase activity and S-adenosylmethionine-dependent methyltransferase activity were also identified.Further analysis indicated that N-methyltransferase(NMT)is mainly involved in alkaloid biosynthesis and O-methyltransferase(OMT)participates in the regulation of coumarin accumulation in wampee.This suggested that wampee's richness in alkaloids and coumarins might be due to the gene expansions of NMT and OMT.The tandem repeat event was one of the major reasons for the NMT expansion.Hence,the reference genome of C.lansium will facilitate the identification of some useful medicinal compounds from wampee resources and reveal their biosynthetic pathways.

关 键 词：Clausena lansium GENOME EVOLUTION methyltransferase activity alkaloid biosynthesis coumarin accumulation 

分 类 号：S567.239[农业科学—中草药栽培]