基于水凝胶表面增强拉曼光谱(SERS)基底检测研究进展  

Research Progress of Detection Based on Hydrogel Surface Enhanced Raman Spectroscopy(SERS)Substrate

作　　者：汪德颖 盛万里 邹明强[1] 裴佳欢 罗云敬[2] 齐小花[1] WANG De-ying;SHENG Wan-li;ZOU Ming-qiang;PEI Jia-huan;LUO Yun-jing;QI Xiao-hua(Chinese Academy of Inspection and Quarantine,Beijing 100123,China;Department of Environment and Life,Beijing University of Technology,Beijing 100124,China;Hohhot Customs and Technology Center,Huhhot 010020,China)

机构地区：[1]中国检验检疫科学研究院,北京100123 [2]北京工业大学环境与生命学部,北京100124 [3]呼和浩特海关技术中心,内蒙古呼和浩特010020

出　　处：《光谱学与光谱分析》2024年第10期2701-2708,共8页Spectroscopy and Spectral Analysis

基　　金：国家重点研发计划项目(2022YFF0607903);检科院基本科研业务费项目(2022JK18);海南省重点研发计划项目(ZDYF2021SHFZ069);国家药监局体外快速诊断试剂技术转化与控制重点实验室资助。

摘　　要：表面增强拉曼光谱(SERS)具有超高灵敏度、指纹信息、少量样本和无损检测等独特优势,设计和制备具有优异重现性和稳定性的SERS基底是实现SERS检测技术进一步发展的关键因素。水凝胶是一种新型的封装材料,其交联聚合物网络具有能够保留大量水分的三维分层结构,并且对杂质具有良好的阻隔作用和强大的抗干扰能力。水凝胶SERS基底具有低成本、高灵敏度、快速检测、高通量等诸多优点,在这篇综述中,主要从SERS基底研究进程,水凝胶SERS基底优点,水凝胶SERS基底在食品、生物、环境检测领域的应用三个方面进行综述,以期为水凝胶SERS基底制备提供新的参考。首先,针对SERS基底研究进程,早期的固定金属纳米粒子(MNPs)刚性固态基底中,MNPs倾向于氧化和聚集,SERS基底重现性差,无法对表面粗糙样品进行分析;柔性支撑材料上修饰MNPs的SERS基底中,MNP s检测过程中容易分离,SERS基底灵敏度和稳定性差。为获得具有良好均匀性和稳定性的S ERS基底,将水凝胶与等离子体纳米结构结合,水凝胶作为MNPs的保护层为SERS分析提供了可靠的尺寸和电荷选择性,同时保持了高渗透性。其次,水凝胶SERS基底可以无需样品预处理对待测物实现原位检测,生物相容性复合水凝胶可以直接体内检测,DNA水凝胶可以精确识别,修饰多重抗体的水凝胶可以同时检测多种分析物,可调网孔尺寸的微凝胶对目标物具有选择筛分作用,三维纳米结构的水凝胶提供更多吸附热点。目前,制备的水凝胶SERS微粒、芯片、贴片等在食品安全、生物医药,环境监测的现场痕量分析方面显示出巨大的潜力。综上,水凝胶SERS基底的制备具有很好的发展前景,能够为今后的分析检测领域提供新的借鉴。Surface-enhanced Raman spectroscopy(SERS)has unique advantages such as ultra-high sensitivity,fingerprint information,small samples,and non-destructive detection.The design and preparation of a SERS substrate with excellent reproducibility and stability are key factors in the further development of SERS detection technology.Hydrogel is a new type of encapsulation material;its cross-linked polymer network has a three-dimensional layered structure that can retain a large amount of water and has a good blocking effect on impurities and strong anti-interference ability.Hydrogel SERS substrate has many advantages,such as low cost,high sensitivity,rapid detection,and high throughput.In this review,the research process of SERS substrate,the advantages of hydrogel SERS substrate,and the application of hydrogel SERS substrate in the field of food,biology,and environmental detection are mainly reviewed to provide a new reference for the preparation of hydrogel SERSsubstrate.First,for the SERS substrate research process,early fixed metal nanoparticles(MNPs)rigid solid substrate,precious MNPs tend to oxidate and aggregate.SERS substrate has poor reproducibility,so it cannot analyze the surface rough samples.In the SERS substrate with MNPs modified on the flexible support material,MNPs are easy to separate in the detection process,and the sensitivity and stability of the SERS substrate are poor.The hydrogel is combined with a plasma nanostructure to obtain a SERS substrate with good uniformity and stability.Hydrogel,as the protective layer of MNPs,provides reliable size and charge selectivity for SERS analysis while maintaining high permeability.Furthermore,hydrogel SERS substrate can achieve in situ detection of the target without sample pretreatment,biocompatible composite hydrogels can be directly detected in vivo,and DNA hydrogel substrate can be accurately recognized,hydrogel modified with multiple antibodies allow for the detection of multiple analytes simultaneously,microgel with adjustable mesh size have a selective s

关 键 词：水凝胶 表面增强拉曼光谱 基底 快速检测 

分 类 号：O657.37[理学—分析化学]