9种转基因玉米外源基因核酸质谱检测方法研究  

PCR MALDI-TOF MS Detection Methods for Nine Exogenous Genes in Transgenic Maize

作　　者：卢小雨 张郁 吕文刚 郭惠民 向才玉 郭悠然 章桂明 LU Xiao-yu;ZHANG Yu;LÜWen-gang;GUO Hui-min;XIANG Cai-yu;GUO You-ran;ZHANG Gui-ming(Animal&Plant Inspection and Quarantine Technology Centre,Shenzhen Customs District,Shenzhen 518010;Zhejiang Digena Diagnostic Technology Co.,Ltd.,Hangzhou 311103;Foshan Customs Integrated Technology Center,Shunde 528303,China)

机构地区：[1]深圳海关动植物检验检疫技术中心,广东深圳518010 [2]浙江迪谱诊断技术有限公司,杭州311103 [3]佛山海关综合技术中心,广东顺德528303

出　　处：《玉米科学》2024年第7期40-45,共6页Journal of Maize Sciences

基　　金：海关总署科研项目(2021HK170)。

摘　　要：针对目前转基因玉米高通量快速筛查技术缺乏,首次利用飞行时间核酸质谱技术,建立pCaMV35S、pFMV35S、tNOS、NPTII、BAR、PAT、CP4-EPSPS、CTP2-CP4-EPSPS和CryIA(b)9种转基因玉米常见外源基因及玉米内源基因ZEIN一次性快速筛查检测方法,靶基因检测限最低为20拷贝。研究建立的方法特异性强、稳定性好、灵敏度高,可用于进口玉米及其产品中转基因成分的快速筛查,为缩短检测周期和提高商品通关速度提供便利。In response to the current lack of high-throughput rapid screening methods for transgenic maize,this study used PCR MALDI-TOF mass spectrometry to realize one-time rapid screening test for nine common exog⁃enous genes pCaMV35S,pFMV35S,tNOS,NPTII,BAR,PAT,CP4-EPSPS,CTP2-CP4-EPSPS,CryIA(b)and the endogenous gene(ZEIN)of transgenic maize.The detection limit for the target genes was as low as 20 copies per re⁃action.The method demonstrated high specificity,stability,sensitivity and accuracy,it can be used for qualitative detection of transgenic components in imported maize and its products.It may improve the speed of commodity cus⁃toms clearance,and provide reference for establishing similar detection methods for other genes and events of trans⁃genic crops.

关 键 词：玉米 外源基因 质谱分析 转基因检测 

分 类 号：S513.035.3[农业科学—作物学]