Soil microbial ecology through the lens of metatranscriptomics  

作　　者：Jingjing Peng Xi Zhou Christopher Rensing Werner Liesack Yong-Guan Zhu 

机构地区：[1]State Key Laboratory of Nutrient Use and Management,College of Resources and Environmental Sciences,National Academy of Agriculture Green Development,Key Laboratory of Plant-Soil Interactions,Ministry of Education,China Agricultural University,Beijing 100193,China [2]Institute of Environmental Microbiology,College of Resource and Environment,Fujian Agriculture and Forestry University,Fuzhou 350002,China [3]Max Planck Institute for Terrestrial Microbiology,Marburg 35043,Germany [4]State Key Laboratory of Urban and Regional Ecology,Research Centre for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China [5]Key Laboratory of Urban Environment and Health,Institute of Urban Environment,Chinese Academy of Sciences,Xiamen 361021,China

出　　处：《Soil Ecology Letters》2024年第3期1-15,共15页土壤生态学快报（英文）

摘　　要：Metatranscriptomics is a cutting-edge technology for exploring the gene expression by,and functional activities of,the microbial community across diverse ecosystems at a given time,thereby shedding light on their metabolic responses to the prevailing environmental conditions.The double-RNA approach involves the simultaneous analysis of rRNA and mRNA,also termed structural and functional metatranscriptomics.By contrast,mRNA-centered metatranscriptomics is fully focused on elucidating community-wide gene expression profiles,but requires either deep sequencing or effective rRNA depletion.In this review,we critically assess the challenges associated with various experimental and bioinformatic strategies that can be applied in soil microbial ecology through the lens of functional metatranscriptomics.In particular,we demonstrate how recent methodological advancements in soil metatranscriptomics catalyze the development and expansion of emerging research fields,such as rhizobiomes,antibiotic resistomes,methanomes,and viromes.Our review provides a framework that will help to design advanced metatranscriptomic research in elucidating the functional roles and activities of microbiomes in soil ecosystems.

关 键 词：METATRANSCRIPTOMICS MRNA MAGs rhizobiont RESISTOME virome 

分 类 号：S154.3[农业科学—土壤学]