基于铁配合物的焦磷酸根离子荧光探针的构建及生物成像  

Construction and Bioimaging of PPi Fluorescent Probes Based on Iron Complexes

作　　者：陈壹刘 宗洪凤 李锦 高妍[1] 肖嘉扬 高云[1] CHEN Yiiu;ZONG Hongfeng;LI Jin;GAO Yan;XIAO Jiayang;GAO Yun(College of Chemical Engineering,University of Science and Technology Liaoning,Anshan 114051,China)

机构地区：[1]辽宁科技大学化学工程学院,辽宁鞍山114051

出　　处：《发光学报》2024年第9期1578-1586,共9页Chinese Journal of Luminescence

基　　金：国家自然科学基金(U1910215);辽宁省科技厅项目(LNTP20231014607);国家级大学生创新创业训练计划项目(202310146036);辽宁省大学生创新创业训练计划项目(S202310146066);辽宁科技大学大学生创新创业训练计划项目(X202310146220)。

摘　　要：以萘并[2,1-b]呋喃-2-碳酰肼和4-甲酰基-3-羟基-N-丁基-1,8-萘二甲酰亚胺为原料经缩合反应制备了一种新型配体ARC,配体ARC与Fe^(3+)按照1∶1络合制备了荧光探针ARC-Fe^(3+),通过高分辨率质谱(HR-MS)、核磁共振氢谱(1H NMR)对探针结构进行了表征。利用紫外-可见吸收光谱、荧光光谱等方法对ARC-Fe^(3+)的识别性能进行了研究。结果表明,探针ARC-Fe^(3+)可通过荧光“OFF-ON”高选择、高灵敏地识别焦磷酸根离子(PPi),探针ARC-Fe^(3+)识别PPi的检测限为1.12×10^(-8) mol/L,且可在60 s内完成对PPi的响应。探针ARC-Fe^(3+)已被成功应用于细胞和活鼠体内对PPi的荧光成像研究。A novel ligand ARC was synthesized by condensation reaction of naphthalene[2,1-b]furan-2-carbohy-drazide and 4-formyl-3-hydroxy-N-butyl-1,8-naphthalenediimide.The ligand ARC was synthesized by 1∶1 complex reaction with Fe^(3+).It was studied by high-resolution mass spectroscopy(HR-MS),NMR hydrogen spectroscopy(1H NMR),UV spectroscopy,and fluorescence spectroscopy.The results showed that the probe ARC-Fe^(3+)can identify pyrophosphate with high selectivity and sensitivity through fluorescence“OFF-ON”.The detection limit of the probe ARC-Fe^(3+)to identify pyrophosphate is 1.12×10^(-8) mol/L,and the response to pyrophosphate can be completed within 60 s.The probe ARC-Fe^(3+)has been successfully applied to the fluorescence imaging of pyrophosphate in cells and living mice,so it can be applied to various fields such as food,medicine,medical treatment and environment in the future.

关 键 词：Fe^(3+)配合物 荧光探针 PPI 细胞成像 活鼠成像 

分 类 号：O657.3[理学—分析化学] TS207.3[理学—化学]