肾衰泄浊汤对慢性肾脏病并发动脉粥样硬化模型大鼠肠道菌群的影响  

Effect of Shenshuaixiezhuo Decoction on Intestinal Microflora in Ratswith Chronic Kidney Disease Complicated with Atherosclerosis

作　　者：刘庚鑫 张格第 袁文萋 罗富里 晏子友[2] LIU Gengxin;ZHANG Gedi;YUAN Wenqi;LUO Fuli;YAN Ziyou(Jiangxi University of Traditional Chinese Medicine Nanchang,Nanchang 330000,China;The Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine Nanchang,Nanchang 330000,China)

机构地区：[1]江西中医药大学临床医学院,江西南昌330000 [2]江西中医药大学附属医院肾病科,江西南昌330000

出　　处：《湖北民族大学学报（医学版）》2024年第3期1-6,共6页Journal of Hubei Minzu University(Medical Edition)

基　　金：2022年度国家自然科学基金(82260908);江西省2023年度研究生创新专项资金项目(YC2023-B225)。

摘　　要：目的探讨肾衰泄浊汤调节肠道菌群干预慢性肾脏病(CKD)合并动脉粥样硬化(AS)的作用机制。方法将30只SD大鼠随机分为3组,分别为假手术组、模型组、肾衰泄浊汤组,除假手术组外其余各组通过5/6肾切除同时给于高脂饮食复制CKD合并AS大鼠动物模型。肾衰泄浊汤组中药汤液按12.0g/(kg·d)剂量灌胃,假手术组与模型组给予等体积生理盐水灌胃,持续8周后处死取材。全自动生化分析仪检测大鼠血肌酐(Scr)、尿素氮(BUN)、总胆固醇(TC)、甘油三酯(TG)水平;HE染色后行主动脉组织病理学观察;16S rDNA测序研究各组大鼠肠道菌群结构。结果与模型组相比,肾衰泄浊汤组大鼠Scr、BUN、TC和TG水平显著降低(P<0.01)。主动脉组织学观察显示肾衰泄浊汤组较模型组病理变化减轻。16S-rDNA测序表明,肾衰泄浊汤可调节CKD合并AS大鼠肠道菌群多样性和组成,降低菌群失调指数(P<0.05),与模型组相比,肾衰泄浊汤提高了厚壁菌门拟杆菌门(B/F)比值,降低了致病菌葡萄球菌属(Staphylococcus)和棒状杆菌属(Corynebacterium)的丰度,升高了假丝酵母菌属(candidatus saccharimonas)的丰度(P<0.05),LEfse分析肾衰泄浊汤组的特征细菌是经粘液真杆菌、双歧杆菌、丹毒丝菌等。结论肾衰泄浊汤可通过调节肠道菌群组成干预CKD合并AS的发生发展。Objective To investigate the effect of Shenshuaixiezhuo Decoction on regulating intestinal flora in chronic kidney disease(CKD)complicated with atherosclerosis(AS).Methods 30 SD rats were randomly divided into 3 groups,which were sham operation group,model group and Shenshuaixiezhuo Decoction group.Except sham operation group,the other groups were given 5/6 nephrectomy and high-fat diet to replicate CKD complicated with AS rat model.Shenshuaixiezhuo Decoction group was given 12.0 g/(kg·d)traditional Chinese medicine decoction by intragastric administration,sham operation group and model group were given equal volume normal saline intragastric administration,which lasted for 8 weeks.The levels of blood creatinine(Scr),urea nitrogen(BUN),total cholesterol(TC),triglyceride(TG)were measured by automatic biochemical analyzer.HE staining was used to observe the histopathology of aorta.The intestinal flora structure of rats in each group was studied by 16S rDNA sequencing.Results Compared with model group,Scr,BUN,TC and TG levels in Shenshuaixiezhuo Decoction group were significantly decreased(P<0.01).Histological observation of aorta showed that the pathological changes of Shenshuaixiezhuo Decoction were less than that of model group.16S rDNA sequencing showed that Shenshuaixiezhuo Decoction could regulate the diversity and composition of intestinal flora in CKD complicated with AS rats,and reduce the number of health disorders of the flora(P<0.05).Compared with the model group,Shenshuaixiezhuo Decoction increased the ratio of firmicutes to Bacteroides.The abundance of pathogenic bacteria Staphylococcus and Corynebacterium decreased,while the abundance of candidatus saccharimonas increased(P<0.05).LEfse analysis of the characteristic bacteria of Shenshuaixiezhuo Decoction group were Eubacterium mucosus,bifidobacterium,erysipelothrix,etc.Conclusion Shenshuaixiezhuo Decoction can interfere with the development of CKD As by regulating the composition of intestinal flora.

关 键 词：肠道菌群 慢性肾脏病 动脉粥样硬化 肾衰泄浊汤 

分 类 号：R289[医药卫生—方剂学]