可变色ELISA样品稀释液制备及其性能检测  

Dilution preparation and performance testing of colorable ELISA samples

作　　者：董林[1,2] 谢金文 孟卫芹[1] 王艳萍 刘吉山[1] 王金良[1] 曲光刚[1] DONG Lin;XIE Jinwei;MENG Weiqin;WANG Yanping;LIU Jishan;WANG Jinliang;QU Guanggang(Binzhou Animal Science and Veterinary Medicine Institute,Binzhou 256600,China;Shandong Lvdu Bio-Sciences&Technology Co.,Ltd.,Binzhou 256600,China)

机构地区：[1]山东省滨州畜牧兽医研究院,山东滨州256600 [2]山东绿都生物科技有限公司,山东滨州256600

出　　处：《畜牧与兽医》2024年第10期57-62,共6页Animal Husbandry & Veterinary Medicine

基　　金：山东省自然科学基金资助项目(ZR2023MC212);山东省现代农业产业技术体系牛创新团队滨州综合试验站项目(SDAIT-09-12)。

摘　　要：为解决酶联免疫吸附法(ELISA)检测过程中样品错加、漏检问题,研制开发了一种可变色ELISA试剂盒样品稀释指示剂,明确了其制备方法,并开展了性能检测评价及其在检测过程的应用特性。可变色稀释指示剂制备方法为:在0.01 mol/L PBS(pH=7.2)基础缓冲液中,加入血清蛋白组分、0.01%可变色指示剂和特效稳定剂,充分溶解后再添加Krovin750防腐剂,定容后即可。结果:制备的可变色ELISA样品稀释显色反应明显,可用于ELISA试剂盒检测中的血清或血浆样本稀释,有效避免检测过程样品跳孔和错加现象;同时对样品具有良好保护,显著提高了检测结果的准确性和稳定性,实现了对样品的有效保护,降低了样品基质背景干扰,提高了检测结果的准确性和稳定性。In order to solve the problem of sample mis-addition and missed detection in the ELISA detection process,a color changing ELISA kit sample dilution indicator was developed to clarify its preparation method,conduct performance testing evaluation,and its application characteristics in the detection process.The dis-colorable dilution indicators consisted of basic buffer,serum protein components,discolorable indicator,special stabilizer,high-efficiency antibacterial and preservative,and purified water.The preparation method was to first prepare a 0.01 mol/L PBS(pH=7.2)base buffer solution;then serum protein components,0.01%color changing indicator,and special stabilizer were added.After sufficient dissolution,Krovin750 preservative was added,and after constant dissolution,the ELISA kit sample dilution indicator was obtained.The results showed that the prepared dis-colorable ELISA sample exhibited obvious color reaction at dilution,and could be used for diluting serum or plasma samples in ELISA kit detection,effectively avoiding sample jumping and mis-addition in the detection process.At the same time,this method had good protection for the sample,significantly improving the accuracy and stability of the detection results,achieving effective protection for the sample,reducing the background interference of the sample matrix,and improving the accuracy and stability of the detection results.

关 键 词：可变色 ELISA 样品稀释液 性能评价 

分 类 号：S854[农业科学—临床兽医学]