1株高致病性基因2型猪流行性腹泻病毒毒株的分离鉴定  

Isolation and characterization of a highly pathogenic genotype 2 porcine epidemic diarrhea virus strain

作　　者：罗毅 徐发荣 徐莹 张乐乐 王郁捷 尹灵丹 刘平黄[1] LUO Yi;XU Farong;XU Ying;ZHANG Lele;WANG Yujie;YIN Lingdan;LIU Pinghuang(College of Veterinary Medicine,China Agricultural University,Beijing 100193,China;Beijing Animal Disease Prevention and Control Center,Beijing 102600,China)

机构地区：[1]中国农业大学动物医学院,北京100193 [2]北京市动物疫病预防控制中心,北京102600

出　　处：《畜牧与兽医》2024年第10期94-100,共7页Animal Husbandry & Veterinary Medicine

基　　金：国家重点研发计划项目(2021YFD1801104)。

摘　　要：为了研究猪流行性腹泻病毒(PEDV)流行毒株的生物学特性,采集仔猪临床腹泻病料进行病毒分离,结果显示,接种病料的Vero细胞连续盲传3代后可观察到明显的细胞病变,通过间接免疫荧光试验确定导致细胞病变的病毒为PEDV,毒株命名为JMS。通过RT-PCR扩增JMS毒株S基因并进行系统进化树分析发现,JMS属于GⅡb基因型。采用500 TCID50(半数组织细胞感染量)的剂量对3日龄仔猪进行攻毒发现,攻毒仔猪表现出严重的临床症状,并在48 h内死亡,致死率为100%。对仔猪剖检和病理组织切片检查发现,攻毒仔猪肠壁变薄,绒毛结构破损、变短甚至脱落。综上,本研究分离到1株高致病性PEDV毒株,为后续探究PEDV流行毒株生物学特性以及相关疫苗研发奠定了基础。To study the biological characteristics of the circulating strain of porcine epidemic diarrhea virus(PEDV),Vero cells were used to isolate the virus from piglet diarrheal samples,and the results showed that cytopathies could be obviously observed after three consecutive blind passages of Vero cells inoculated with the diseased material,and the virus that led to the cellular lesions was determined to be PEDV(named JMS)by indirect immunofluorescence assay.The S gene of the JMS strain was amplified by RT-PCR,and the phylogenetic tree analysis revealed that JMS belonged to the GⅡb genotype.Then,the 3-day-old piglets were attacked with a dose of 500 TCID50,and it was found that the attacked piglets showed severe clinical symptoms and died within 48 h,with a lethality rate of 100%.Subsequent autopsy and histopathological examination of the piglets revealed that the intestinal wall of the poisoned piglets became thinner,and the villi structure was damaged,shortened or even detached.These results indicated that a highly pathogenic strain of PEDV was isolated in this study,which laid a foundation for investigation of the biological characteristics of the clinically prevalent strain of PEDV and for the development of related vaccines.

关 键 词：猪流行性腹泻病毒 GⅡb型 高致病性 

分 类 号：S852[农业科学—基础兽医学]