基于量子点的非洲猪瘟病毒抗体荧光免疫层析试纸研制  

Establishment of a quantum dots-based immunochromatographic strip for detection of African swine fever virus specific antibodies

作　　者：孙亚宁[1] 杨苏珍[1] 邢云瑞 范璐[1] 乔松林[1] 卢清侠[1] 张改平[1] SUN Yaning;YANG Suzhen;XING Yunrui;FAN Lu;QIAO Songlin;LU Qingxia;ZHANG Gaiping(Key Laboratory of Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

机构地区：[1]河南省农业科学院动物免疫学重点实验室,河南郑州450002

出　　处：《畜牧与兽医》2024年第10期101-108,共8页Animal Husbandry & Veterinary Medicine

基　　金：河南省自然科学基金青年基金项目(232300421274);河南省农业科学院优秀青年科技基金项目(2022YQ25);河南省重点研发与推广专项(科技攻关)(222102110413);“中原英才计划”(育才系列)-中原青年拔尖人才项目(ZYYCYU202012148)。

摘　　要：为了研发灵敏、准确的非洲猪瘟快速诊断试纸产品,本研究基于免疫层析技术原理,以非洲猪瘟病毒(ASFV)p72蛋白三聚体为检测抗原,量子点(QDs)为标记材料,金黄色葡萄球菌A蛋白(SPA)为检测线,p72单克隆抗体为质控线,建立了非洲猪瘟病毒抗体荧光免疫层析试纸,并对试纸的检测性能进行鉴定。结果:荧光免疫层析试纸检测ASFV标准阳性血清的效价为1∶102400,与猪瘟病毒、口蹄疫病毒、伪狂犬病病毒、猪繁殖与呼吸道综合征病毒及猪圆环病毒2型阳性血清抗体无交叉反应,在临床样本检测中与商业化ASFV抗体检测试剂盒的符合率为92.92%。综上,该试纸具有良好的灵敏度、特异性、准确性及稳定性,可用于临床样品中ASFV抗体的快速筛查。Based on the principle of immunochromatography,water-soluable quantum dots(QDs)were used as the labeling material conjugated with the trimer of ASFV protein,Staphylococcus aureus protein A and ASFV p72 monoclonal antibody immobilized on the strip were used as the detection line and quality control line,respectively,and a sensitive and accurate test strip for rapid detection of African swine fever was established in this study.The results showed that the sensitivity of the immunochromatographic test strip was 1∶102400,with no cross-reaction with antibody positive sera of swine fever virus,swine foot-and-mouth disease virus,pseudorabies virus,porcine reproductive and respiratory syndrome virus and porcine circovirus type 2.The total coincidence rate of the strip with the commercial ELISA kit was 92.92%.This strip had good sensitivity,specificity,accuracy and stability,which might be used for rapid screening of African swine fever virus specific antibodies.

关 键 词：非洲猪瘟 量子点 p72蛋白 抗体 试纸 

分 类 号：S854[农业科学—临床兽医学]