Bulked Segregant RNA-Seq Analysis of Pollinated Pistils Reveals Genes Influencing Spikelet Fertility in Rice  

作　　者：Kanokwan KAEWMUNGKUN Keasinee TONGMARK Sriprapai CHAKHONKAEN Numphet SANGARWUT Theerachai THANANANTA Amorntip MUANGPROM 

机构地区：[1]Department of Biotechnology,Faculty of Science and Technology,Thammasat University,Rangsit Centre,Khlong Luang,Pathum Thani 12120,Thailand [2]National Center for Genetic Engineering and Biotechnology,National Science and Technology Development Agency,Thailand Science Park,Khlong Luang,Pathum Thani 12120,Thailand

出　　处：《Rice science》2024年第5期556-571,I0031-I0056,共42页水稻科学（英文版）

基　　金：supported by the Agricultural Research Development Agency of Thailand (Grant No.PRP6405030280);Research Promotion fund for International and Educational Excellence, Thailand (Grant No.08/2562)。

摘　　要：Prezygotic isolation is important for successful fertilization in rice, significantly affecting yield. This study focused on F_(5:6) generation plants derived from inter-subspecific crosses(Nipponbare × KDML105) with low(LS) and high seed-setting rates(HS), in which normal pollen fertility was observed. However, LS plants showed a reduced number of pollen grains adhering to the stigma and fewer pollen tubes reaching the ovules at 4-5 h post-pollination, compared with HS plants. Bulked segregant RNA-Seq analysis of pollinated pistils from the HS and LS groups revealed 249 and 473 differentially expressed genes(DEGs), respectively. Kyoto Encyclopedia of Genes and Genomes analysis of the HS and LS-specific DEGs indicated enrichment in metabolic pathways, pentose and glucuronate interconversions, and flavonoid biosynthesis. Several of these DEGs exhibited co-expression with pollen development genes and formed extensive clusters of co-expression networks. Compared with LS pistils, enzyme genes controlling pectin degradation, such as OsPME35 and OsPLL9, showed similar expression patterns, with higher levels in HS pistils pre-pollination. Os02g0467600, similar to cinnamate 4-hydroxylase gene(CYP73), involved in flavonoid biosynthesis, displayed higher expression in HS pistils post-pollination. Our findings suggest that OsPME35, OsPLL9, and Os02g0467600 contribute to prezygotic isolation by potentially modifying the stigma cell wall(OsPME35 and OsPLL9) and controlling later processes such as pollen-stigma adhesion(Os02g0467600) genes. Furthermore, several DEGs specific to HS and LS were co-localized with QTLs and functional genes associated with spikelet fertility. These findings provide valuable insights for further research on rice spikelet fertility, ultimately contributing to the development of high-yielding rice varieties.

关 键 词：bulked segregant RNA-Seq flavonoid inter-subspecific cross PECTIN pollinated pistil prezygotic barrier spikelet fertility 

分 类 号：S511[农业科学—作物学]