基于WGCNA方法挖掘与猪皮下脂肪沉积相关的重要基因及通路  

Identification of Key Genes and Pathways Involved in Subcutaneous Fat Deposition in Pigs Based on WGCNA Method

作　　者：熊霞飞 陈强 XIONG Xiafei;CHEN Qiang(College of Animal Science and Technology,Yunnan Agricultural University,Kunming Yunnan 650201,China)

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《现代畜牧科技》2024年第9期39-43,共5页Modern Animal Husbandry Science & Technology

基　　金：云南农业大学科研启动基金项目(KY2022-31)。

摘　　要：脂肪性状是养猪业最重要的经济性状之一。该研究基于基因表达公共数据库GEO数据库中瘦肉型猪(长白猪)和脂肪型猪(荣昌猪)皮下脂肪组织的基因表达数据集GSE30343,利用加权基因共表达网络分析等方法挖掘与猪脂肪沉积相关的重要基因和信号通路。结果显示,共识别出4个基因共表达模块,其中,宝蓝色模块为与皮下脂肪最显著相关模块(r=0.94,P=5E-17),并识别出8个与脂肪形成相关的重要基因,分别为GPAT、ACACA、ACLY、SCD、FASN、ACSS2、ME1和SCD5,这些基因主要参与有机酸代谢合成、脂肪酸代谢合成、AMPK等信号通路。该研究挖掘出了与不同脂肪类型猪脂肪沉积相关的重要基因,为利用分子手段对猪脂肪性状进行遗传改良提供了潜在候选基因。Fat trait is one of the most important economic traits in pig industry.In this study,based on the gene expression data set GSE30343 of subcutaneous adipose tissue of lean pigs(Landrace pigs)and fatty pigs(Rongchang pigs)in the public GEO database.Weighted gene co-expression network analysis and other methods were used to explore key genes and signaling pathways related to pig fat deposition.The results showed that four gene co-expression modules were identified,among which the turquoise module was the most significant correlation module(r=0.94,P=5E-17)and eight key genes related to fat formation were identified.Eight key genes are GPAT,ACACA,ACLY,SCD,FASN,ACSS 2,ME1 and SCD5,respectively.These genes are mainly involved in organic acid metabolism synthesis,fatty acid metabolism synthesis,AMPK and other signaling pathways.This study found some important genes related to fat deposition between different fat types of pigs,which provided potential candidate genes for genetic improvement of fat trait in pigs by molecular technology.

关 键 词：脂肪沉积 皮下组织 WGCNA 基因 

分 类 号：S828[农业科学—畜牧学]