星点设计—效应面法优化巯基壳聚糖的合成  

Synthesis Optimization of Thiolated Chitosan based on Central Composite Design-Response Surface Method

作　　者：白雪宁 刘丹丹 余婷 王馨悦 赵伟奇 景少璠 BAI Xuening;LIU Dandan;YU Ting;WANG Xingyue;ZHAO Weiqi;JING Shaofan(School of Biomedical and Chemical Engineering,Liaoning Institute of Science and Technology,Benxi Liaoning 117004,China)

机构地区：[1]辽宁科技学院生物医药与化学工程学院,辽宁本溪117004

出　　处：《辽宁科技学院学报》2024年第4期36-39,共4页Journal of Liaoning Institute of Science and Technology

基　　金：辽宁科技学院大学生创新创业训练计划项目“功能性壳聚糖衍生物的开发及其应用探索”(202411430092);辽宁省教育厅高等学校基本科研项目(重点项目)(2024JYTZD-01).

摘　　要：应用星点设计-效应面法优化巯基壳聚糖的合成条件,以提高产物游离巯基含量,获得生物学性能较高的巯基化壳聚糖。以N-乙酰-L-半胱氨酸与壳聚糖的比例以及反应液pH值为主要因素,以产物的游离巯基含量为评价指标,进行模型预测和验证。二项式拟合方程具有较好的预测性,优化所得聚合物的游离巯基含量为(505.4±9.8)μmol/g,与预测值偏差较小。结果表明,星点设计-效应面法能够较好地优化巯基壳聚糖的合成条件,所得巯基壳聚糖具有较高的游离巯基含量和黏膜黏附性。In order to obtain the product with high biological properties,the synthesis conditions of thiolated chitosan was optimized based on central composite design-response surface method.The ratio of N-acetyl-L-cysteine to chitosan and pH value of reaction solution were selected as the main factors,and the content of free thiol content of the product was used as the evaluation index to predict and verify the model.The results showed the binomial fitting equation has good predictability and the free thiol content of the optimized polymer is(505.4±9.8)μmol/g,which is close to the predicted value.Also,the central composite design-response surface method can optimize the synthesis conditions of thiolated chitosan,and the obtained polymer has high content of free sulfhydryl group and mucosal adhesion.

关 键 词：巯基壳聚糖 N-乙酰-L-半胱氨酸 星点设计-效应面法 

分 类 号：X832[环境科学与工程—环境工程] O657.32[理学—分析化学]