杉木与浙江楠混交对根系分泌物和丛枝菌根真菌群落结构的影响  

Effects of Mixed Plantation of Cunninghamia lanceolata and Phoebe chekiangensis on Root Exudates and Community Structure of Arbuscular Mycorrhizal Fungi

作　　者：杨淑雅 王镜如 朱滢滢 伊力塔[1] 刘美华[1] Yang Shuya;Wang Jingru;Zhu Yingying;Yi Lita;Liu Meihua(College of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou 311300)

机构地区：[1]浙江农林大学林业与生物技术学院,杭州311300

出　　处：《林业科学》2024年第9期59-68,共10页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(31971623)。

摘　　要：【目的】研究杉木与浙江楠混交对根系分泌物和土壤中丛枝菌根真菌(AMF)群落结构的影响,为杉木人工纯林“针改阔”的树种选择提供参考,为提高杉-阔混交林生产力提供理论依据和思路。【方法】在杉木纯林、浙江楠纯林、杉木与浙江楠混交林中,采集0~20 cm土层样品,分析纯林和混交林土壤中根系分泌物和AMF群落结构差异,阐明混交林根系分泌物对AMF群落结构变化的影响。【结果】纯林与混交林土层样品中共检出含量高且具有化感活性的主要分泌物5种:2,4-二叔丁基苯酚、邻苯二甲酸二丁酯、油酸甲酯、棕榈酸、硬脂酸,其中油酸甲酯、棕榈酸、硬脂酸含量在不同林分间差异显著。浙江楠纯林根际土壤中含AMF属的OTU数量最多、多样性和相对丰度最高,混交林次之,杉木纯林最低;3种林分土壤AMF优势属均为Glomus属,混交显著降低Glomus占比、提高Gigaspora占比。相关分析表明,邻苯二甲酸二丁酯对杉木纯林土壤AMF群落结构影响较大,油酸甲酯对混交林土壤AMF群落结构影响较大。【结论】杉木与浙江楠混交使土壤化感物质含量发生改变,影响土壤微生物结构组成,AMF群落多样性和丰富度显著高于杉木纯林。【Objective】This study aims to examine the effects of mixed plantation of Cunninghamia lanceolata and Phoebe chekiangensis on root exudates and community structure of arbuscular mycorrhizal fungi(AMF).The findings would provide theoretical reference for the management and productivity improvement of C.lanceolata plantations.【Method】In a pure C.lanceolata forest,a pure of P.chekiangensis forest,and a mixed C.lanceolata and P.chekiangensis forest,soil samples were collected from 0–20 cm soil layer to analyze the differences in root exudates and AMF community structure in the pure and mixed forests,and to elucidate the effects of root exudates in the mixed forest on the community structure of AMFs.【Result】Five main types of exudates with high content and allelopathic activity,2,4-di-tert-butylphenol,dibutyl phthalate,methyl oleate,palmitic acid,and stearic acid,were detected in the soil samples from pure and mixed forests.Among them,the contents of methyl oleate,palmitic acid,and stearic acid varied significantly among different forest types.The number of operational taxonomic units(OTUs)and relative abundance of genera at the OTU level were the highest in the soil of pure P.chekiangensis forest,followed by mixed forest,and the lowest in pure C.lanceolata forest.The dominant genus of AMF in the soils of all three forests was Glomus.The percentage of Glomus was significantly reduced and the percentage of Gigaspora was increased in the mixed forest.Correlation analysis showed that dibutyl phthalate had a greater effect on soil AMF community structure in the C.lanceolata pure forest,and methyl oleate had a greater effect on soil AMF community structure in the mixed forest.【Conclusion】The mixed plantation of C.lanceolata and P.chekiangensis results in changes in the content of allelochemicals and affects soil microbiome composition,resulting in significantly higher AMF community diversity and richness compared to pure Chinese fir forest.The results of this study can provide references for the selection

关 键 词：混交林 根系分泌物 丛枝菌根真菌 杉木 浙江楠 纯林 

分 类 号：Q958.15[生物学—动物学] S154.36[农业科学—土壤学] S154.4[农业科学—农业基础科学]