饱和烷烃处理下紫花苜蓿(Medicago sativa)的转录学特征  

Analysis of transcriptome data of Medicago sativa treated with saturated alkanes

作　　者：李岩[1,2] 李云昊 李雅茹 赵敏 秦天宇[4] 王洪粤 黄萱 LI Yan;LI Yunhao;LI Yaru;ZHAO Min;QIN Tianyu;WANG Hongyue;HUANG Xuan(National Engineering Laboratory for Exploration and Development of Low Permeability Oil and Gas Fields,Xi’an 710018,China;Changqing Oilfield Oil and Gas Technology Research Institute,Xi’an 710018,China;Shaanxi Provincial Key Laboratory of Biotechnology,Xi’an 710069,China;School of Life Sciences,Northwest University,Xi’an 710069,China)

机构地区：[1]低渗透油气田勘探开发国家工程实验室,陕西西安710018 [2]长庆油田油气工艺研究院,陕西西安710018 [3]陕西省生物技术重点实验室,陕西西安710069 [4]西北大学生命科学学院,陕西西安710069

出　　处：《西北大学学报（自然科学版）》2024年第5期889-898,共10页Journal of Northwest University（Natural Science Edition）

基　　金：国家自然科学基金(31300223);陕西省自然科学基金(2016JM3001);低渗透油气田勘探开发国家工程实验室开放课题(KFKT2023-04)。

摘　　要：为探究紫花苜蓿在石油污染下的耐受机理,采用超声碎促溶的方法,将3种有机物(十二烷、十六烷和二十四烷)配置成质量分数均为1%的混合溶液,模拟饱和烷烃污染对紫花苜蓿幼苗进行处理,分别对污染0,6,24 h的植株取样进行转录组学分析,共获得1431个差异表达基因(DEGs)。GO富集分析表明,这些DEGs主要涉及蛋白结合、代谢途径和催化活性等;KEGG富集分析表明,DEGs主要富集到植物病原体相互作用、MAPK信号通路和光合生物碳固定途径等。qRT-PCR验证转录组结果可靠。研究结果为研究植物降解和耐受原油中饱和石油烃污染机制原理及后续筛选和培育耐石油污染植物提供理论依据。To explore the tolerance mechanism of Medicago sativa under oil pollution,this study adopted the method of ultrasonic crushing and solubilization to prepare a mixed solution of three organic compounds(Dodecane,n-Hexadecane,and n-Tetracosane)at a concentration of 1%to simulate saturated alkanes and treat Medicago sativa seedlings.Samples were taken from plants exposed to pollution for 0h,6h,and 24h for transcriptomic analysis.The results showed that a total of 1431 DEGs were obtained.GO enrichment analysis indicated that these DEGs were mainly involved in protein binding,metabolic pathways,and catalytic activity.KEGG enrichment analysis showed that DEGs were mainly enriched in plant pathogen interaction,MAPK signaling pathway and photosynthetic biological carbon fixation pathway.qRT-PCR was further used to verify the reliability of the results.This study provides theoretical basis for exploring the mechanism of plant degradation and tolerance of saturated petroleum hydrocarbon in crude oil pollution,as well as subsequent screening and cultivation of petroleum-tolerant plants through transcriptome analysis of Medicago sativa treated with saturated alkanes.

关 键 词：石油烃污染 紫花苜蓿 转录组 差异表达基因 

分 类 号：S541[农业科学—作物学]