支气管哮喘患儿继发肺部感染的肠道菌群特征  

Intestinal flora of bronchial asthma children with secondary pulmonary infection

作　　者：张海涛 王玉 石苗苗 袁丽萍[2] 胡波[2] 杨泽玉 ZHANG Hai-tao;WANG Yu;SHI Miao-miao;YUAN Li-ping;HU Bo;YANG Ze-yu(Children's Hospital of Anhui Province,Hefei,Anhui 23005l,China;不详)

机构地区：[1]安徽省儿童医院呼吸科,安徽合肥230051 [2]安徽医科大学第一附属医院儿科,安徽合肥230022

出　　处：《中华医院感染学杂志》2024年第18期2780-2784,共5页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金资助项目(81471617)。

摘　　要：目的探讨支气管哮喘患儿继发肺部感染的肠道菌群结构及功能特征。方法选取2022年2月-2024年2月安徽省儿童医院呼吸科收治的29例哮喘继发肺部感染患儿为感染组,随机选取同期入院治疗的31例单纯哮喘患儿为非感染组,检测两组患儿炎症细胞因子白细胞介素(IL)-1β、IL-2R、IL-6、IL-8、IL-10和肿瘤坏死因子-α(TNF-α)水平,使用宏基因组高通量技术对两组患儿新鲜粪便标本进行肠道菌群测序并进行生物信息分析。结果感染组患儿炎症细胞因子IL-1β、IL-2R、IL-6、IL-8和TNF水平高于非感染组(P<0.05),肠道菌群的α多样性和β多样性显著降低(P<0.05);两组在属水平上的优势菌为拟杆菌属(Bacteroides)、普雷沃菌属(Prevotella)、另枝菌属(Alistipes)和真杆菌属(Eubacterium)等,其中感染组Prevotella、巨单细胞菌属(Megamonas)、阿克曼菌属(Akkermansia)和坦纳菌属(Tannerella)菌群相对丰度较高,非感染组中副拟杆菌属(Parabacteroides)、小类杆菌属(Dialister)、小类杆菌属(Faecalibacterium)、瘤胃球菌属(Ruminococcus)和Alistipes相对丰度较高;两组差异优势功能基因主要集中在新陈代谢(包括外源物质的生物降解和代谢、能量代谢、脂质代谢等)方面。结论支气管哮喘患儿继发肺部感染血清炎症细胞因子水平升高,且肠道微生物多样性及物种组成均发生了改变。OBJECTIVE To explore the structure and functional characteristics of intestinal flora of the bronchial asthma children with secondary pulmonary infection.METHODS A total of 29 bronchial asthma children with secondary pulmonary infection who were treated in respiratory medicine department of Anhui Province Children′s Hospital from Feb 2022 to Feb 2024 were assigned as the infection group,meanwhile,31 children with simple asthma who were hospitalized for treatment were chosen as the non-infection group.The levels of inflammatory cytokines including interleukin(IL)-1β,IL-2R,IL-6,IL-8,IL-10 and tumor necrosis factor-α(TNF-α)were detected for the two groups of children.Metagenomic high-throughput technique was employed to carry out the sequencing for the intestinal flora from fresh stool specimens that were collected from the two groups of children.RESULTS The levels of inflammatory cytokines IL-1β,IL-2R,IL-6,IL-8 and TNF of the infection group were higher than those of the non-infection group(P<0.05).Theαdiversity andβdiversity of intestinal flora were remarkably lower in the infection group than in the non-infection group(P<0.05).Bacteroides,Prevotella,Alistipes and Eubacterium were dominant among the bacteria on genus level;the relative abundance of flora Prevotella,Megamonas,Akkermansia and Tannerella was higher in the infection group than in the non-infection group;while the relative abundance of Parabacteroides,Dialister,Faecalibacterium,Ruminococcus and Alistipes was higher in the non-infection group than in the infection group.The major difference in the dominant functional genes between the two groups was the metabolism,including biodegradation and metabolism of allogenic material,energy metabolism and lipid metabolism.CONCLUSION The bronchial asthma children with secondary pulmonary infection show the rise of serum inflammatory cytokines and change of diversity of intestinal microorganisms and species composition.

关 键 词：支气管哮喘 肺部感染 肠道菌群 肠-肺轴 宏基因组学 细胞因子 

分 类 号：R725.6[医药卫生—儿科]