基于非靶向代谢组学的G80烟叶成熟进程的代谢特征  

Metabolic characterisation in maturation process of flue-cured tobacco variety G80 based on untargeted metabolomics

作　　者：钟汝力 卢向阳[1] 刘璐 谢向丽 刘雄伟 范伟 周喜新[1] ZHONG Ruli;LU Xiangyang;LIU Lu;XIE Xiangli;LIU Xiongwei;FAN Wei;ZHOU Xixin(Collegeof Biological Science and Technology,Hunan Agricultural University,Changsha,Hunan 410128,China;College of Food Science and Technology,Hunan Agricultural University,Changsha,Hunan410128,China)

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]湖南农业大学食品科学技术学院,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2024年第4期21-27,共7页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省烟草公司长沙市公司科技项目(21-23A05);湖南中烟工业有限责任公司科技项目(KY2020JD0008)。

摘　　要：运用超高效液相色谱-串联质谱联用技术,从烤烟品种G80不同成熟期(打顶当天及打顶后第10、20、30天)烟叶中鉴定出亲水性代谢物211种、亲脂性代谢物74种;共筛选出94个差异代谢物,归属于氨基酸、糖及糖苷类、脂类、胺类、生物碱、苯丙酸及其衍生物、萜类、核苷酸和其他类等9大类,其中L-苯丙氨酸、DL-苯丙氨酸、DL-酪氨酸、蔗糖和甘油酸为G80烟叶成熟过程中的关键代谢物。代谢通路分析发现,G80不同成熟期烟叶差异代谢物均显著富集于苯丙氨酸代谢途径,其代谢产物木脂素、苯丙酸、黄酮类和香豆素及其衍生物等在成熟后期大量积累,有助于增强烟叶的抗氧化能力和促进香气的形成。Two hundred and elevenhydrophilic metabolites and 74 lipophilic metabolites from tobacco leaves of flue-cured tobacco variety G80 at different maturity stages(on the day of topping and on the 10th,20th and 30th daysof topping)were identified using ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).A total of 94 differential metabolites were screenedout,including nine major groups:amino acids,sugars and glycosides,lipids,amines,alkaloids,phenylalanine and its derivatives,terpenes,nucleotides,and others.The key metabolites in tobacco leavesduring the maturation process of flue-cured tobacco varietyG80 were found to be L-phenylalanine,DL-phenylalanine,DL-tyrosine,sucroseandglyceric acid.Metabolic pathway analysis showed that the differential metabolites of tobacco leavesin flue-cured tobacco varietyG80 at different maturation stages were significantly enriched in the phenylalanine metabolic pathway.Metabolites such as lignans,phenylpropanoic acid,flavonoids,and coumarins and their derivatives,which were accumulated in large quantities during the late stage of maturation,could improve the antioxidant capacity and aroma formation of the tobacco leaves.

关 键 词：烤烟G80 成熟进程 代谢组学 差异代谢物 苯丙氨酸代谢 

分 类 号：S572.01[农业科学—烟草工业]