基于转录组与代谢组料慈竹的耐盐机制分析  

Salt tolerance mechanism analysis of Bambusa distegiabased on transcriptome and metabolome

作　　者：杜娟 陈咸吉 王哲 聂现辉 陈彦超 艾文胜[3] 孟勇[3] 汪启明[1,2] 彭国平 DU Juan;CHEN Xianji;WANG Zhe;NIE Xianhui;CHEN Yancao;AI Wensheng;MENG Yong;WANG Qiming;PENG Guoping(College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha,Hunan 410128,China;Hunan Engineering Laboratory for Good Agricultural Practice and Comprehensive Utilization of Famous-Region Medicinal Plants,Changsha,Hunan 410128,China;Institute of Bamboo and Wood,Hunan Academy of Forestry,Changsha,Hunan 410011,China)

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]道地药用植物规范化栽培与综合利用湖南省工程实验室,湖南长沙410128 [3]湖南省林业科学院竹木研究所,湖南长沙410011

出　　处：《湖南农业大学学报（自然科学版）》2024年第4期35-41,共7页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家重点研发计划(2018YFD0600102)。

摘　　要：选取盐处理组(1.0%NaCl溶液浇灌)和对照组(用井水浇灌)料慈竹的成熟叶片进行转录组与代谢组分析,分析料慈竹在转录水平与次生代谢物水平的变化。结果表明:与对照组相比,处理组共检测到差异基因487个,其中上调基因257个,下调基因230个;GO功能分析基因差异表达(DEGs)结果显示,在盐胁迫下叶片中DNA出现损伤,甲基转移酶复合物富集最多;DEGs的KEGG富集分析发现,料慈竹的糖类、氨基酸类代谢相关途径富集种类较多,次生代谢产物途径富集数量最多;盐处理后差异表达的次生代谢物共有225种,上调的有77种,下调的有148种,其中差异表达显著上调的次生代谢产物1种(log_(2)(FC)≥1),为葫芦巴碱,下调的有20种(log_(2)(FC)≤-1),主要为酚酸类及黄酮类化合物;料慈竹在长时间的盐胁迫下依靠自身的甲基化复合物相关基因调控稳定了基因的表达,但盐胁迫仍然导致DNA受到损伤;通过初生代谢能力的提高及下游苯丙素类生物合成途径的减少,料慈竹的可溶性糖、可溶性蛋白、葫芦巴碱等渗透压调节物质含量提高,维持了渗透压,保证在盐胁迫下的生理活性,提高了在含盐环境中的生长能力。The mature leaves of the salt treatment group with 1.0%NaCl solution and the control group with well water were selected for transcriptome and metabolome analysis to analyze the changes in the transcriptome and secondary metabolite levels of Bambusa distegiainthetwostudiedmaterial.The results showed that compared withthe control group,a total of 487 expressed genes were detected withvariationin the treatment group,including 257 up-regulated genes and 230 down-regulated genes.GO functional analysis of DEGs showed that DNA damage and methyltransferase complexes occurred in the leaves of Bambusa distegiaunder salt stress.The KEGG enrichment analysis of DEGs revealed that more enriched metabolic pathways related to carbohydrate and amino acid metabolism were found in Bambusa distegia,with the largest number of enriched secondary metabolite biosynthetic pathways.With salt treatment,a total of 225 differential secondary metabolites were identified,with 77 up-regulated and 148 down-regulated.Among them,one significantly differential secondary metabolite was up-regulated(log_(2)(FC)≥1),which was trigonelline,and 20 were down-regulated(log_(2)(FC)≤-1)and were mainly phenolic acids and flavonoids.Under prolonged salt stress,methylation complex regulation in the expressed genes of Bambusa distegiamight stabilize gene expression,but salt stress still caused DNA damages in Bambusa distegia.By improving the primary metabolic capacity and reducing the downstream phenylpropanoid biosynthesis pathway,the contents of osmotic pressure regulating substances such as soluble sugar,soluble protein,and trigonelline in Bambusa distegiaincreased,maintaining osmotic pressure and ensuring physiological activity under salt stress.

关 键 词：料慈竹 盐胁迫 代谢组 转录组 苯丙素类生物合成 基因差异表达 

分 类 号：S795[农业科学—林木遗传育种] Q786[农业科学—林学]