大口黑鲈烂身病病原菌的分离鉴定及其药物敏感性  

Isolation,identification and drug sensitivityanalysis of pathogenic bacteria causingrotten disease in Micropterussalmoides

作　　者：冯兴浪 丁戈野 张秋实 侯金亮 刘智明 陈中元 向建国[1] 肖调义[1] 李军华 FENG Xinglang;DING Geye;ZHANG Qiushi;HOU Jinliang;LIU Zhiming;CHEN Zhongyuan;XIANG Jianguo;XIAO Tiaoyi;LI Junhua(Fisheries College,Hunan Agricultural University,Changsha,Hunan 410128,China;College of Life and Environmental Science,Hunan University of Arts and Science,Changde,Hunan 415000,China)

机构地区：[1]湖南农业大学水产学院,湖南长沙410128 [2]湖南文理学院生命与环境科学学院,湖南常德415000

出　　处：《湖南农业大学学报（自然科学版）》2024年第4期82-88,共7页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(32173021);湖南省自然科学基金项目(2021JJ30326);省部共建国家重点实验室开放课题基金(2020KF005);湖南省重点实验室开放课题基金(2021KJ01)。

摘　　要：从湖南省醴陵市某大口黑鲈工厂化养殖场中患烂身病的大口黑鲈体表溃疡处分离得到1株病原菌MS315509,经形态学和分子生物学鉴定,确定该病原菌为嗜水气单胞菌(Aeromonas hydrophila)。人工感染结果显示,MS315509对大口黑鲈鱼苗的半数致死剂量为5.47×10^(7)cfu/mL,为弱毒株;MS315509携带细胞兴奋性肠毒素基因(alt)、细胞毒性肠毒素基因(act)和黏附素基因(aha1)3种毒力基因;采用12种抗生素与12种中草药对菌株MS315509进行药物敏感性试验,结果表明MS315509对庆大霉素、头孢拉定、石榴皮、苏木、五倍子、诃子等高度敏感,对四环素、头孢唑啉、卡那霉素、苯唑西林、恩诺沙星、青霉素、强力霉素、链霉素、黄连、艾叶和熟地黄等表现出耐药性。A bacterial pathogen strain MS315509 was isolated from the surface ulcer ofthe sick Micropterus salmoides(M.salmoides)in a perch farm in Liling City,Zhuzhou City,Hunan Province.The isolate was identified morphologically and molecularly as Aeromonas hydrophila.The results of artificial infection showed that the half lethaldosage of MS315509 inM.salmoidesfry was 5.47×10^(7)cfu/mL.The virulence gene detection showed that the strain MS315509 carried three virulence genes:cytotoxic enterotoxin(alt),excitatory enterotoxin(act)and adhesin(aha1).Drug susceptibility testing of strain MS315509 utilizing 12 antibiotics and 12 herbs showed that the strain MS315509 was highly sensitive to gentamicin,cefradine,Shiliupi,Sumu,Wubeizi,Hezi,and resistant to tetracycline,cefazolin,kanamycin,oxacillin,enrofloxacin,penicillin,vibramycin,streptomycin,Huanglian,Aiyeand Shudihuang.

关 键 词：大口黑鲈 烂身病 嗜水气单胞菌 毒力基因 药物敏感性 致病性 

分 类 号：S941.429[农业科学—水产养殖]