辣蓼黄酮缓解猪伪狂犬病病毒诱导的小鼠炎症及相关信号通路研究  

Study on the Ethyl Acetate Fraction of Flavonoids from Polygonum hydropiper L.Alleviates PRV-induced Inflammation and Related Signalling Pathways in Mice

作　　者：唐敏 谢小东 蒋丽群 童艳梅 任春芝 易首利 施君[2] 胡庭俊[2] TANG Min;XIE Xiao-dong;JIANG Li-qun;TONG Yan-mei;REN Chun-zhi;YI Shou-li;SHI Jun;HU Ting-jun(Department of Clinical Laboratory,the Second Affiliated Hospital of Army Medical University,Chongqing 400037,China;College of Animal Science and Technology,Guangxi University,Nanning,Guangxi 530005,China;Xuzhou Polytechnic College of Biological Engineering,Xuzhou,Jiangsu 221000,China)

机构地区：[1]陆军军医大学第二附属医院检验科,重庆400037 [2]广西大学动物科学技术学院,广西南宁530005 [3]徐州生物工程职业技术学院,江苏徐州221000

出　　处：《动物医学进展》2024年第10期27-33,共7页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(32072907);南宁市科学研究与技术开发计划项目(20232039)。

摘　　要：为研究辣蓼黄酮乙酸乙酯部分(ethyl acetate of flavonoids from Polygonum hydropiper L,FEA)对猪伪狂犬病病毒(Pseudorabies virus,PRV)感染小鼠诱导的炎症反应及炎症相关信号通路的调节作用,以50~200 mg/kg体重的FEA处理PRV感染的小鼠炎症模型后,检测各组小鼠免疫器官指数变化,脾脏匀浆液中氧化应激相关因子、炎症相关因子的水平或表达量,以及NF-κB等炎症相关信号通路的活化情况。结果显示,50和200 mg/kg体重的FEA能降低PRV感染的小鼠脾脏指数(P<0.05);50~200 mg/kg体重FEA能不同程度的降低PRV感染的小鼠脾脏细胞中ROS、NO水平,IL-1β、IL-6、MCP-1和TNF-α等细胞因子的含量及mRNA表达,以及XOD、MPO、iNOS的酶活性(P<0.05),升高IL-10的水平和GSH的酶活性(P<0.05)。此外,FEA降低了PRV感染的小鼠脾脏细胞中p-p65/p65、p-JNK/JNK和p-p38/p38等炎症相关信号通路蛋白的磷酸化比值(P<0.05)。结果表明,FEA能减缓PRV诱导的小鼠氧化应激状态和炎症反应,发挥体内抗炎作用,其可能是通过抑制NF-κB和MAPK等炎症相关信号通路的活化发挥作用。In order to investigate the regulatory effects of ethyl acetate fraction of flavonoids from Polygonum hydropiper L.(FEA)on inflammation and inflammatory signaling pathways induced by pseudorabies virus(PRV)infection in mice,PRV-infected mice inflammatory model was treated with FEA of 50-200 mg/kg body weight,the changes of immune organ indexes,the levels or expressions of oxidative stress-related factors,inflammation-related enzymes and factors in spleen homogenate,as well as the activation of NF-κB and other inflammation-related signaling pathways were detected.The results showed that FEA of 50 and 200 mg/kg body weight could reduce the spleen index of PRV-infected mice(P<0.05);50-200 mg/kg body weight FEA can lower the levels of ROS and NO,the contents or mRNA expressions of IL-1β,IL-6,MCP-1,TNF-αand other cytokines in spleen cells of PRV-infected mice,as well as the enzyme activities of XOD,MPO,iNOS(P<0.05),while the level of IL-10 and enzyme activities of GSH were increased(P<0.05).In addition,FEA decreased the phosphorylation ratios of inflammatory signaling pathway proteins such as p-P65/p65,p-JNK/JNK,and p-P38/p38 in PRV-infected mouse spleen cells(P<0.05).The results showed that FEA can relieve the oxidative stress and inflammatory response induced by PRV in mice,and play an anti-inflammatory role in vivo,possibly by inhibiting the activation of inflammation-related signaling pathways such as NF-κB and MAPK.

关 键 词：辣蓼黄酮 猪伪狂犬病病毒 氧化应激 炎症 核因子-κB 

分 类 号：S853.74[农业科学—临床兽医学]