4种根际促生菌对菘蓝生长与生理的影响  

Effects of four plant growth-promoting rhizobacteria on growth and physiology of Isatis indigotica

作　　者：鲍婷婷 孙莉琼 李晓帆 李宇峰 唐晓清[1] 王康才[1] BAO Tingting;SUN Liqiong;LI Xiaofan;LI Yufeng;TANG Xiaoqing;WANG Kangcai(College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;Suzhou Juxintang Biotechnology Limited Company,Changshu 215500,China;Suzhou Qifan Agricultural Technology Limited Company,Changshu 215500,China)

机构地区：[1]南京农业大学园艺学院,江苏南京210095 [2]苏州聚鑫堂生物科技有限公司,江苏常熟215500 [3]苏州启帆农业科技有限公司,江苏常熟215500

出　　处：《南京农业大学学报》2024年第5期854-863,共10页Journal of Nanjing Agricultural University

基　　金：园艺作物资源与保存研究项目(KYZZ2022004);中药全产业链创新研究项目(KYCXJC2022001);横向合作-中药材种植技术与基地建设指导项目(20231020)。

摘　　要：[目的]本文旨在研究4种不同根际促生菌对菘蓝根际环境改变、生长及生理的影响,为菘蓝绿色生产提供理论参考。[方法]采用4种根际促生菌(肠杆菌GS-22、芽胞杆菌GS-27、鞘氨醇杆菌GS-72和不动杆菌GS-103)单施的方法开展盆栽试验,测定土壤理化指标、菘蓝生长指标、光合指标、营养物质与相关酶活性以及活性成分等,评价根际促生菌对菘蓝的促生效果。[结果]4种根际促生菌对土壤理化性质有不同程度影响,GS-22和GS-72能够提高土壤全氮含量,GS-22和GS-103显著提高土壤速效磷含量,各处理土壤有机质含量均显著提高。接种根际促生菌,土壤脲酶、土壤碱性磷酸酶和土壤蔗糖酶活性较不接种土壤根际菌(CK)的增幅分别为33.78%~73.70%、49.51%~83.93%和8.10%~186.26%。4种根际促生菌促进菘蓝生长,增加株高、主根直径、干重、鲜重等,其中GS-22对菘蓝生长性状影响最显著。根际促生菌对菘蓝生理也有显著影响,各处理菘蓝硝酸还原酶、蔗糖合成酶、酸性磷酸酶活性较CK分别增加53.81%~125.19%、57.64%~76.54%和7.87%~22.78%。4种根际菌有利于菘蓝营养物质(可溶性糖、游离氨基酸)与活性成分(总黄酮、靛蓝、靛玉红)的积累,其中GS-22与GS-27处理均可提高总黄酮、靛蓝和靛玉红的含量。[结论]菘蓝根际促生菌GS-22、GS-27、GS-72和GS-103具促生能力,能够改良土壤环境,对菘蓝的生长与生理过程有促进作用,其中GS-27的综合效果最佳。所筛选的菌株可为开发菘蓝专用微生物菌肥、实现药材增产增效提供策略。[Objectives]The paper aimed to study the effects of four kinds of different plant growth-promoting rhizobacteria(PGPR)on the rhizosphere environment change,growth and physiology of Isatis indigotica,in order to provide theoretical reference for the production of I.indigotica.[Methods]The four kinds of PGPR,which were named Enterobacter sp.GS-22,Bacillus sp.GS-27,Sphingobacterium sp.GS-72 and Acinetobacter sp.GS-103,were applied in pot experiment.In order to evaluate the growth promotion effect of PGPR on I.indigotica,the relevant indicators were determined,including soil physical and chemical properties,growth traits,photosynthetic parameters,nutrient and related enzyme activities,active components,and so on.[Results]The four kinds of PGPR had different effects on soil physicochemical properties.GS-22 and GS-72 could increase soil total nitrogen content,GS-22 and GS-103 significantly increased soil available phosphorus content,and soil organic matter content in all treatment groups significantly increased.Compared with not inoculated with soil rhizosphere bacteria(CK),the activities of soil urease,soil alkaline phosphatase and soil sucrase increased by 33.78%-73.70%,49.51%-83.93%and 8.10%-186.26%,respectively.Four rhizosphere bacteria promoted the growth of I.indigotica,and increased plant height,taproot diameter,dry weight,fresh weight,and so on,and GS-22 had the most significant effect on the growth traits of I.indigotica.PGPR also had significant effects on physiology of I.indigotica.The activities of nitrate reductase,sucrose synthetase and acid phosphatase increased by 53.81%-125.19%,57.64%-76.54%and 7.87%-22.78%compared with CK,respectively.Four rhizosphere bacteria were beneficial to the accumulation of nutrients,like soluble sugars,free amino acids,and active components,like total flavonoids,indigo and indirubin of I.indigotica,in which GS-22 and GS-27 especially increased the contents of total flavonoids,indigo and indirubin.[Conclusions]The rhizosphere bacteria GS-22,GS-27,GS-72 and GS-103 had the abili

关 键 词：菘蓝 根际促生菌 根际环境 促生能力 生长 生理 

分 类 号：S154.3[农业科学—土壤学] S567.2[农业科学—农业基础科学]