龙眼果实采后贮藏期间糖代谢及相关基因表达的分析  

Analysis of sugar metabolism and expression of related genes of longan fruit during the postharvest storage

作　　者：帅良 庞纪伟 胡美华 廖玲燕 殷菲胧 刘云芬 李小洁 何妹英 SHUAI Liang;PANG Jiwei;HU Meihua;LIAO Lingyan;YIN Feilong;LIU Yunfen;LI Xiaojie;HE Meiying(School of Chemistry and Food Science,Nanchang Normal University,Nanchang 330023,China;School of Food and Bioengineering/Guangxi Key Laboratory of Health Care Food Science and Technology,Hezhou University,Hezhou 542899,China;College of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou 545006,China)

机构地区：[1]南昌师范学院化学与食品科学学院,江西南昌330023 [2]贺州学院食品与生物工程学院/广西康养食品科学与技术重点实验室,广西贺州542899 [3]广西科技大学生物与化学工程学院,广西柳州545006

出　　处：《南京农业大学学报》2024年第5期979-988,共10页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(31860457,32260611);南昌师范学院博士启动基金项目(NSBSJJ2023002)。

摘　　要：[目的]本文旨在探究采后常温(RT)和低温(LT)贮藏过程中龙眼果实糖含量和代谢相关酶活性及基因表达变化的规律,为龙眼果实采后贮藏保鲜提供参考依据。[方法]以‘储良’龙眼为试验材料,设置4℃和25℃处理,探究其可溶性固形物(TSS)、蔗糖、葡萄糖和果糖含量变化情况及相关糖代谢酶活性和相关基因表达量的变化规律。[结果]不同贮藏温度下龙眼果实的糖含量变化不同,常温贮藏过程中蔗糖含量下降,葡萄糖和果糖含量上升,而低温贮藏保持较高的蔗糖含量,促进葡萄糖和果糖含量快速下降。与常温贮藏相比,低温贮藏提高了蔗糖磷酸合成酶(SPS)活性,抑制中性转化酶(NI)、酸性转化酶(AI)、蔗糖合成酶(SS)、果糖激酶(FRK)和己糖激酶(HXK)活性,抑制DlNI、DlAI、DlSPS、DlFRK和DlHXK基因的表达。相关性分析表明,低温贮藏改变了龙眼果实中蔗糖含量与糖代谢相关酶活性和基因表达的关系。主成分分析也显示,低温贮藏延缓了龙眼果实蔗糖代谢的发生,保持较好的果实品质。[结论]低温贮藏通过调控龙眼果实糖代谢相关基因的表达,影响相关酶活性进而使糖组分发生变化,使龙眼果实保持较高的蔗糖含量,从而延缓龙眼果实品质劣变。[Objectives]The purpose of this paper was to investigate the changes in sugar content and metabolism-related enzyme activities and gene expression of longan fruits during postharvest storage at room temperature(RT)and low temperature(LT),and to provide a reference basis for postharvest storage and preservation of longan fruits.[Methods]Taking‘Chuliang’longan as the test material,4℃and 25℃treatments were set to investigate the changes of total soluble solids(TSS),sucrose,glucose and fructose contents,and the change rules of related sugar metabolism enzyme activities and related gene expressions.[Results]Sugar content of longan fruit varied differentially at different storage temperatures.Sucrose content decreased,glucose and fructose content increased during RT storage,while LT storage maintained high sucrose content,glucose and fructose content decreased rapidly.Compared with RT storage,sucrose phosphate synthase(SPS)activity increased,neutral invertase(NI),acid invertase(AI),sucrose synthase(SS),fructokinase(FRK)and hexokinase(HXK)activities were inhibited and the expression of DlNI,DlAI,DlSPS,DlFRK,DlHXK genes was inhibited at LT storage.Correlation analysis showed that the relationship about sucrose content,sugar metabolism-related enzyme activities and gene expression were altered in longan fruits at LT storage.In addition,principal component analysis also indicated that LT storage delayed the onset of sucrose metabolism in longan fruit and maintained better fruit quality.[Conclusions]By regulating the expression of genes related to sugar metabolism in longan fruits,low temperature storage affected the activity of related enzymes and then changed the sugar components,so that longan fruits maintained high sucrose content and delayed the quality deterioration of longan fruits.

关 键 词：龙眼 糖代谢 酶活性 基因表达 相关性分析 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]