复合益生菌酸马奶对AD大鼠记忆功能及肠道菌群的影响  

Effect of compound probiotic sour mare milk on memory function and intestinal flora in AD rats

作　　者：伊再提古丽·外力 卡丽比奴尔·艾尔肯 徐志伟 马健宝 古丽巴哈尔·卡吾力[1] Yizaitiguli·Waili;Kalibinuer·Aierken;XU Zhiwei;MA Jianbao;Gulibahaer·Kawuli(College of Pharmacy,Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学药学院,乌鲁木齐830011

出　　处：《中国乳品工业》2024年第9期5-13,21,共10页China Dairy Industry

基　　金：新疆维吾尔自治区重大科技专项项目(2022A02013-3);新疆天然药物活性组分与释药技术重点实验室项目(XJDX1713)。

摘　　要：文章探讨了复合益生菌酸马奶对AD大鼠的缓解作用及肠道菌群的影响。72只SD大鼠,随机分为正常对照组、模型组、阳性对照组(甘露特纳胶囊)、复合益生菌酸马奶高、中、低剂量组并建立AD大鼠模型。持续灌胃60 d并收集粪便供16S rRNA测序;检测大鼠行为学变化、海马CA1区神经元形态变化及海马区BDNF蛋白表达量;检测血清5-羟色胺(5-HT)、γ-氨基丁酸(GABA)、白细胞介素-1β、白细胞介素-18、肿瘤坏死因子-α的浓度。与模型组比较,阳性对照组和复合益生菌酸马奶高剂量组有效区域进入次数显著增加,逃避潜伏期缩短,原平台所在象限滞留时间百分比增加;阳性对照组和复合益生菌酸马奶各剂量组神经细胞受损程度低于模型组;与模型组相比,阳性对照组和复合益生菌酸马奶高剂量组AD大鼠海马区BDNF蛋白和血清5-HT、GABA含量显著增加,IL-1β、IL-18、TNF-α显著降低;阳性对照组、复合益生菌酸马奶高剂量组的肠道菌群多样性与正常对照组接近,复合益生菌酸马奶对海马体的学习及记忆力有积极影响。Seventy-two SD rats were randomly divided into normal control group,model group,positive control group(Ganoderma capsules),and high,medium and low dose groups of probiotic yogurt and established AD rat model.The rats were gavaged for 60 days and the faeces were collected for 16S rRNA sequencing;the behavioural changes of the rats and the morphological changes of neurons in the hippocampal CA1 area were examined.The expression of BDNF protein in the hippocampal region of the rat brain was measured;the concentrations of serum 5-hydroxytryptamine,γ-aminobutyric acid,interleukin-1β,interleukin-18 and tumour necrosis factor-αwere measured.Compared with the model group,the positive control group and the high dose group of probiotic yogurt had significantly more effective area entry,shorter escape latency and increased percentage of retention time in the quadrant where the original plateau was located;the degree of neuronal cell damage was lower in the positive control group and each dose group of probiotic yogurt than the model group;compared with the model group,the hippocampal area of AD rats in the positive control group and the high dose group of probiotic yogurt the BDNF protein and serum 5-HT and GABA levels were significantly increased,and IL-1β,IL-18 and TNF-αwere significantly decreased in the positive control group and the high dose group of probiotic yogurt.Probiotic complex yoghurt had a positive effect on learning and memory in the hippocampus.

关 键 词：复合益生菌酸马奶 阿尔茨海默 肠道菌群 

分 类 号：TS252.14[轻工技术与工程—农产品加工及贮藏工程]