离子色谱法测定尿中硝酸盐、硫氰酸盐和高氯酸盐的方法研究  

Determination of nitrate,thiocyanate and perchlorate in urine by ion chromatography

作　　者：朱慧敏 孟元华[1] 龚燕[1] 王昱尘 周小新[1] ZHU Hui-min;MENG Yuan-hua;GONG Yan;WANG Yu-chen;ZHOU Xiao-xin(The Affiliated Wuxi Center for Disease Control and Prevention of Nanjing Medical University,Wuxi Center for Disease Control and Prevention,Wuxi 214023,China;School of Public Health,Nanjing Medical University,Nanjing 211166,China)

机构地区：[1]无锡市疾病预防控制中心(南京医科大学附属无锡市疾病预防控制中心),江苏无锡214023 [2]南京医科大学公共卫生学院,江苏南京211166

出　　处：《化学工程师》2024年第9期26-30,共5页Chemical Engineer

基　　金：无锡市科协软课题(KX-23-C134);无锡市公共卫生中心(LCZX2021006);医学创新团队(CXTD2021004)。

摘　　要：建立离子色谱测定尿中的硝酸盐、硫氰酸盐和高氯酸盐的方法。采用超纯水稀释尿样,RP前处理小柱去除尿中的干扰离子,以AS20阴离子交换柱为分析柱,KOH淋洗液梯度洗脱,电导检测器进行检测。离子色谱法测定尿中硝酸盐、硫氰酸盐和高氯酸盐可在35min内实现基线分离,检出限分别为5.5μg·L^(-1)、2.8μg·L^(-1)和4.0μg·L^(-1);3种物质在线性范围内相关系数(r)均大于0.999;相对标准偏差为0.17%~6.28%;加标回收率为86.1%~107.7%。本实验建立的方法操作简便、准确、灵敏度高,可适用于同时检测尿中硝酸盐、硫氰酸盐和高氯酸盐。To establish an ion chromatography method for the determination of nitrate,thiocyanate and perchlorate in urine.The urine samples were diluted with ultra-pure water,and the interference ions in urine were removed by RP pretreatment column.AS20 anion exchange column was used as analysis column,potassium hydroxide eluent was gradient elution,and conductivity detector was used for detection.The baseline separation of nitrate,thiocyanate and perchlorate could be achieved within 35 min by ion chromatography,and the detection limits were 5.5μg·L^(-1),2.8μg·L^(-1) and 4.0μg·L^(-1).The correlation coefficients(r)of the three substances were all greater than 0.999 in the linear range.The relative standard deviation is 0.17%~6.28%.The recoveries were 86.1%~107.7%.The experimental results show that the method established in this study is simple,accurate and sensitive,and can be used for simultaneous detection of nitrate,thiocyanate and perchlorate in urine.

关 键 词：离子色谱法 尿 硝酸盐 硫氰酸盐 高氯酸盐 

分 类 号：R446.12[医药卫生—诊断学]