儿童手足口病相关微生物群落功能变化研究  

Study on the Functional Changes of Microbial Communities Associated with Hand,Foot,and Mouth Disease in Children

作　　者：金洪伟 冯滨 郑宇航 彭文婷 Jin Hongwei;Feng Bin;Zheng Yuhang;Peng Wenting(Guangming District Center for Disease control and Prevention,Shenzhen,Guangdong,518106,China)

机构地区：[1]深圳市光明区疾病预防控制中心,广东深圳518106

出　　处：《中国初级卫生保健》2024年第9期52-55,共4页Chinese Primary Health Care

基　　金：深圳市光明区软科学研究项目(卫生系统)(2021R01146)。

摘　　要：目的:通过对手足口病患儿与健康儿童的宏基因组学进行深入研究,揭示手足口病与微生物群落结构及功能变化之间的关联。方法:2020—2022年,收集并通过宏基因组学分析儿童手足口病患者及健康对照组的样本,比较不同组之间的微生物组成差异,采用生物信息学工具分析手足口病患儿的宏基因组数据与健康儿童的差异;得出某些病毒性负荷的增加与疾病的严重程度相关联;识别得到与病程发生和发展密切相关的关键基因和代谢途径。结果:手足口病患儿的微生物组成与健康儿童相比存在显著的差异,患儿微生物组中某些病毒负荷显著增加;重症患儿的微生物群落结构和功能与轻症患儿和健康儿童相比也存在明显的差异,表明微生物群落的失衡可能与疾病的严重程度有着密切的联系。重症患儿的微生物群落功能分析揭示了其对宿主免疫系统的潜在抑制作用,或许能为疾病的重症化提供解释。结论:通过对比手足口病患儿与健康儿童的微生物群落,揭示了与发展相关的关键微生物和代谢途径,通过微生物功能预测分析,确认患儿的微生物群落在病毒复制和宿主免疫响应等关键代谢途径上的功能增强,为微生物组干预手足口病的预防和治疗提供新角度。OBJECTIVE To conduct an in-depth metagenomic study on children with hand,foot,and mouth disease(HFMD)and healthy children to reveal the association between HFMD and changes in the structure and function of microbial communities.METHODS Samples from children with HFMD and healthy controls were collected and analyzed using metagenomic approaches.The differences in microbial composition between different groups were compared,and metagenomic data from HFMD patients were analyzed using bioinformatics tools to identify key genes and metabolic pathways closely related to the course of the disease and its development.RESULTS Compared to healthy children,the microbial composition of HFMD patients showed significant differences,with certain viral loads significantly increased in the patients’microbiota.The structure and function of the microbial community in severe HFMD patients also differed significantly from those in mild cases and healthy children,indicating that microbial community imbalance may be closely related to the severity of the disease.Functional analysis of the microbial community in severe HFMD patients revealed a potential suppressive effect on the host immune system,which may explain the severity of the disease.CONCLUSION By comparing the microbial communities of HFMD patients and healthy children,key microbes and metabolic pathways related to the development of HFMD were revealed.Functional prediction analysis confirmed that the microbial communities of HFMD patients have enhanced functions in key metabolic pathways such as viral replication and host immune response,providing a new perspective for the prevention and treatment of HFMD through microbial intervention.

关 键 词：手足口病 宏基因组学 高通量测序 微生物组成 

分 类 号：R725.1[医药卫生—儿科]