3种野生苔草种子萌发特性对不同盐分胁迫的响应  

Responses of Seed Germination Characteristics of Three Wild Carex Species to Different Salt Stresses

作　　者：闫玉邦 白小明[1] 朱雅楠 张才忠 冉福 李娟霞 康瑞卿 李萍 何琳琳 YAN Yu-bang;BAI Xiao-ming;ZHU Ya-nan;ZHANG Cai-zhong;RAN Fu;LI Juan-xia;KANG Rui-qing;LI Ping;HE Ling-ling(College of Pratacultural Science,Gansu Agricultural University,Key Laboratory for Grassland Ecosystem,Ministry of Education,Pratacultural Engineering Laboratory of Gansu Province,Sino-U.S.Centers for Grazing Land Ecosystem Sustainability,Lanzhou,Gansu Province 730070,China)

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃省草业工程实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草地学报》2024年第9期2919-2933,共15页Acta Agrestia Sinica

基　　金：甘肃省科技计划项目(20 JR10RA564);甘肃省林草局草原生态修复治理科技支撑项目(GSLC-2020-3);甘肃省林业和草原局草原生态恢复与管理科技支撑项目(GSLC-2020-3,LCJ20210021)资助。

摘　　要：本研究以3种野生苔草(Carex)种子为材料,用3种单盐(NaCl,Na_(2)SO_(4),NaHCO_(3))及相互组合的4种复盐模拟盐胁迫,采用培养皿法研究不同盐分类型及盐浓度(50,100,150,200 mmol·L^(-1))对野生苔草种子萌发特性和幼苗生长的影响。结果表明:低浓度的NaCl、H_(1)(NaCl和Na_(2)SO_(4)混合)和H_(4)(NaCl,Na_(2)SO_(4)和NaHCO_(3)混合)促进青绿苔草(Carex breviculmis)种子的萌发,低浓度的H_(4)促进无脉苔草(Carex enervis)种子的萌发。50 mmol·L^(-1)Na_(2)SO_(4)促进无脉苔草胚芽的生长,50 mmol·L^(-1)NaCl促进无脉苔草胚根的生长。在单盐或复盐胁迫中,NaCl对野生苔草种子萌发的影响最小,当盐溶液中含有HCO_(3)^(-)离子时,对3种野生苔草种子萌发的胁迫程度较强。隶属函数法综合评价显示,3种野生苔草种子耐盐能力强弱依次为:无脉苔草>异穗苔草>青绿苔草,7种盐分类型对耐盐能力不同的野生苔草种子萌发特性的影响程度的强弱依次为:NaHCO_(3)>H_(3)>H_(2)>H_(4)>H_(1)>Na_(2)SO_(4)>NaCl。In this study,three wild Carex species seeds were used as materials,three salts(NaCl,Na_(2)SO_(4),NaHCO_(3))and four their combinations were used to simulate salt stress.Petri dish method was used to study the effects of different salt types and salt concentrations(50,100,150,200 mmol·L^(-1))on seed germination characteristics and seedling growth of wild Carex.The results showed that low concentrations of NaCl,H_(1)(NaCl and Na_(2)SO_(4)mixture)and H_(4)(NaCl,Na_(2)SO_(4)and NaHCO_(3)mixture)promoted the germination of Carex breviculmis seeds,and low concentration of H_(4)promoted the germination of Carex enervis seeds.50 mmol·L^(-1)Na_(2)SO_(4)promoted the growth of plumule of Carex enervis,and 50 mmol·L^(-1)NaCl promoted the growth of radicle of Carex enervis.Under single salt or compound salts stress,NaCl had the least effect on seed germination in wild Carex,the degree of stress on the seed germination of three wild Carex was stronger when HCO_(3)^(-)was contained in the salt solution.The comprehensive evaluation of the membership function method showed that the salt tolerance of the three wild Carex species seeds was Carex enervis>Carex heterostachya>Carex breviculmis,and the effects of seven salt types on the seed germination characteristics of wild Carex were NaHCO_(3)>H_(3)>H_(2)>H_(4)>H_(1)>Na_(2)SO_(4)>NaCl.

关 键 词：野生苔草 单盐 复盐 萌发特性 盐分类型 

分 类 号：S326[农业科学—作物遗传育种]