湖北紫荆染色体核型特征及rDNA分布分析  

Karyotype Characteristics and rDNA Distribution Analysis of Cercis glabra Pampan.Chromosomes

作　　者：孙雪霞 娄秋莲 马琴[2] 王霞 刘麟 Sun Xuexia;Lou Qiulian;Ma Qin;Wang Xia;Liu Lin(Zhengzhou Park Plaza Transaction Center,Zhengzhou 450000,China;Henan Sesame Research Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

机构地区：[1]郑州市公园广场事务中心,郑州450000 [2]河南省农业科学院芝麻研究中心,郑州450002

出　　处：《河南林业科技》2024年第3期5-8,12,共5页Journal of Henan Forestry Science and Technology

摘　　要：试验以45S和5S rDNA重复序列为探针,采用荧光原位杂交(Fluorescence in situ hybridization,FISH)技术,对湖北紫荆染色体rDNA分布进行物理定位和分析。结果表明,染色体形态观察表明湖北紫荆染色体组中含有3对随体染色体,核型公式为2n=2x=14=14 m,染色体组为1A核型,核型不对称系数为51.10%,染色体长度介于2.37~4.23μm,其长度多为中等染色体(M1和M2类型),长度组成公式为:2L+4M2+8M1。FISH结果表明,有3对(第1、2、3对)染色体携带45S rDNA基因信号位点,2对荧光信号分布在染色体短臂末端,1对荧光信号分布在染色体长臂中端;1对染色体(第6对染色体)携带有5S rDNA基因信号位点,其分布在染色体短臂端部,且45S rDNA和5S rDNA的信号位点不重合。试验结果对湖北紫荆染色体rDNA位点的定位研究,将为该物种的遗传多样性分析和基因组结构解析提供更多关键信息。We performed the karyotypeanalysis and location of rDNA for Cercis glabra Pampan.chromosomes based on probes of 45S and 5S rDNA repeat sequences and the fluorescence in situ hybridization(FISH)technology.The results of chromosome morphology observation showed that the Cercis glabra Pampan.genome contains three pairs accompanying chromosomes,the chromosome karyotype was 2n=2x=14=14m,which belonged to 1A karyotype with 51.10%of the AS.K(asymmetrical karyotype coefficient).The chromosome length ranging from 2.37 to 4.23μm,where most chromosomes are of medium size(M1 and M2 types),and the length composition formula is 2L+4M2+8M1.In addition,the FISH results of rDNA indicated that 3 pairs of chromosomes,including the 1th,2th,and 3th pairs,carried 45S rDNAs,2 pairs and 1 pair fluorescence signals were distributed at the short arms end of the chromosome and the middle end of the long arm of the chromosome,respectively;another pair of chromosomes(the 6th pair)carries 5S rDNA,which distributed at the short arm end of the chromosome,and the signal sites of 45S rDNA and 5S rDNA do not overlap.The results will provide more kye information for genetic diversity and genome structure of Cercis glabra Pampan..

关 键 词：湖北紫荆 染色体 核型分析 荧光原位杂交 RDNA 

分 类 号：S685.99[农业科学—观赏园艺] Q943[农业科学—园艺学]