陈皮活性成分川陈皮素抑制IL-23/IL-17轴抗炎作用研究  

The study of nobiletin from Citri Reticulatae Pericarpium on Anti-inflammatory by inhibiting IL-23/IL-17

作　　者：刘青[1] 刘敬 雷金 郑逸航 伍秋婷 王俊杰[1] 王庆[1] 李玉娟[1] Liu Qing;Liu Jing;Lei Jin(Xiangnan University,Chenzhou,Hunan 423000,China)

机构地区：[1]湘南学院,湖南郴州423000

出　　处：《湘南学院学报（医学版）》2024年第3期1-6,共6页Journal of Xiangnan University（Medical Sciences）

基　　金：湖南省自然科学基金(2024JJ7522);湖南省教育厅科学研究优秀青年项目(23B0771);湖南省卫生健康委科研计划课题(D202302048964);湖南省双一流应用特色学科药学学科(湘教通〔2022〕351号);生物医药产业化中试技术研发公共服务平台(湘南学院科发〔2022〕3号);郴州国家可持续发展议程创新示范区建设省级专项“南岭优势及特色药用植物全产业链关键技术研发”(2023sfq04)。

摘　　要：目的探究川陈皮素(Nobiletin)对脂多糖(lipopolysaccharides,LPS)诱导的小鼠巨噬细胞的抗炎活性及具体作用机制。方法CCK-8法检测川陈皮素对小鼠巨噬细胞的细胞毒性,确定安全用药浓度。采用LPS刺激小鼠巨噬细胞构建细胞炎症模型,同时加入5—80μmol/L的川陈皮素处理24 h,Griess法检测细胞上清液中NO含量,ELISA法检测小鼠巨噬细胞促炎因子(TNF-α和IL-6)的分泌水平,实时荧光定量聚合酶链式反应检测实验细胞中TNF-α、IL-6、IL-23和IL-17A mRNA的表达水平。结果CCK-8结果显示川陈皮素浓度在80μmol/L及以下对细胞活力的影响差异无统计学意义(P>0.05),LPS刺激后细胞上清液中NO含量显著升高(P<0.01),表明细胞炎症模型构建成功。与LPS组相比,川陈皮素处理后,细胞上清液中NO含量显著降低(P<0.01),细胞促炎因子(TNF-α和IL-6)分泌显著减少(P<0.01),TNF-α、IL-6、IL-23和IL-17A mRNA的表达水平显著降低(P<0.01),并呈剂量依赖。结论川陈皮素通过调控IL-23/IL-17炎症轴并抑制TNF-α和IL-6的产生发挥抗炎活性,改善LPS诱导的小鼠巨噬细胞的炎症反应。Objective To study the anti-inflammatory effect and mechanism of on LPS-induced mouse macrophages.Methods CCK-8 method was used to detect the cytotoxicity of nobiletin on mouse macrophages and to determine the safe dosing concentration.LPS was used to stimulate mouse macrophages to construct a cellular inflammation model,and 5-80μmol/L of nobiletin was added for 24 h.The NO content in the cell supernatant was detected by the Griess method and the secretion levels of pro-inflammatory factors(TNF-αand IL-6)in mouse macrophages were detected by ELISA.The mRNA expressions of TNF-α,IL-6,IL23 and IL-17A in experimental cells were tested by RT-PCR.Results CCK-8 results showed that the difference in cell viability was not statistically significant(P>0.05)when the concentration of nobiletin was at 80μmol/L and below,and the NO content in the cell supernatant was significantly elevated(P<0.01)after LPS stimulation,which indicated that the cellular inflammation model was successfully constructed.Compared with the LPS group,nobiletin treatment significantly reduced the NO content in the cell supernatant(P<0.01),the secretion of cellular pro-inflammatory factors(TNF-αand IL-6)(P<0.01),and the expression levels of TNF-α,IL-6,IL-23,and IL-17A mRNA(P<0.01)in a dosage-dependent manner.Conclusion Nobiletin alleviates the LPS-induced inflammatory response of mouse macrophages through regulating the inflammatory axis of IL-23/IL-17 and inhibiting the production of pro-inflammatory factors(TNF-αand IL-6).

关 键 词：川陈皮素 炎症反应 IL-23/IL-17 TNF-Α IL-6 

分 类 号：R285.5[医药卫生—中药学]