甘孜州燕子沟4种高山杜鹃根系真菌群落组成与多样性分析  

Composition and Diversity of Root Fungi of Four Rhododendron lapponicum Species in Yanzigou,Garze Tibetan Autonomous Prefecture

作　　者：刘润宇 李良导[1,3] 杨宇杰 于晓英 许璐[1,2,3] 李炎林 曹福祥 LIU Run-yu;LI Liang-dao;YANG Yu-jie;YU Xiao-ying;XU Lu;LI Yan-lin;CAO Fu-xiang(College of Landscape Architecture and Art Design,Hunan Agricultural University,Changsha 410128,PRC;College of Horticulture,Hunan Agricultural University,Changsha 410128,PRC;Hunan Engineering Research Center for Breeding and Utilization of High-Quality Flowers and Trees in Central Subtropical,Changsha 410128,PRC)

机构地区：[1]湖南农业大学风景园林与艺术设计学院,湖南长沙410128 [2]湖南农业大学园艺学院,湖南长沙410128 [3]湖南省中亚热带优质花木繁育与利用工程研究中心,湖南长沙410128

出　　处：《湖南农业科学》2024年第9期15-20,33,共7页Hunan Agricultural Sciences

基　　金：湖南省科技厅重点研发项目(2017NK2252)。

摘　　要：为促进四川甘孜州燕子沟高山杜鹃的引种驯化与移栽,并探索不同生境条件下根系微生物多样性,采集了羊踯躅、棕背杜鹃、光亮杜鹃、腺房杜鹃4种高山杜鹃的根系,通过高通量测序和生物信息学分析,评估该地区高山杜鹃类型根系真菌的组成和多样性。结果显示:4种高山杜鹃根系样品中共获得有效序列746,973条,进而聚合得到1,101个操作分类单元(OTUs);这些真菌分属于6门、27纲、30目、30科、30属,其中子囊菌门和担子菌门为主要类群,它们在群落丰度上表现出较大的差异;在属级水平上,主要优势菌属差异显著,特别是光亮杜鹃根系真菌群落不仅拥有最多的特异性OTUs,且群落丰度最高;棕背杜鹃的根系真菌群落拥有最少的特异性OTUs,而羊踯躅的根系真菌群落丰度最低。整体而言,该试验区域内4种高山杜鹃的根系真菌多样性,与同一地区低海拔地带相比,显著偏低,这符合高海拔地区真菌多样性减少的普遍趋势。In order to facilitate the introduction,domestication,and transplantation of Rhododendron lapponicum species in Yanzigou,Garze Tibetan Autonomous Prefecture,Sichuan Province,as well as explore the diversity of root microbiota under different habitat conditions,the root systems of four R.lapponicum species including R.lapponicum,R.nivale,R.aganniphum,and R.russatum,were collected to assess the composition and diversity of the root fungi of the alpine R.lapponicum types in this region through highthroughput sequencing and bioinformatics analysis.The results showed that a total of 746973 effective sequences were obtained from the rhizosphere soil samples of the four alpine R.lapponicum species,which were then aggregated into 1101 operational taxonomic units(OTUs).These fungi belonged to 6 phyla,27 classes,30 orders,30 families,and 30 genera,with Ascomycota and Basidiomycota being the dominant phyla,displaying significant differences in community abundance.At the genus level,there were notable differences in the predominant fungal genera,particularly the rhizosphere fungal community of R.aganniphum,which not only had the highest number of unique OTUs but also the greatest community abundance.The rhizosphere fungal community of R.nivale had the fewest unique OTUs,while R.lapponicum had the lowest community abundance.Overall,the diversity of rhizosphere fungi associated with the four R.lapponicum species in the study was significantly lower compared to lower-altitude areas in the same region,which was consistent with the trend of reduced fungal diversity at higher altitudes.

关 键 词：高山杜鹃 高通量测序 根系真菌 群落结构 多样性 

分 类 号：S685.21[农业科学—观赏园艺]