一株具有杀松材线虫活性的马尾松内生菌分离及鉴定  

Isolation and Identification of an Endophyte with Killing Effect on Pine Wood Nematodes from Pinus massoniana

作　　者：黄文静 李珍 夏永刚[2] 谭泽宝 肖琴 朱桓吾 罗坤[1] HUANG Wen-jing;LI Zhen;XIA Yong-gang;TAN Ze-bao;XIAO Qin;ZHU Huan-wu;LUO Kun(College of Plant Protection,Hunan Agricultural University,Changsha 410128,PRC;Hunan Academy of Forestry,Changsha 410018,PRC)

机构地区：[1]湖南农业大学植物保护学院,湖南长沙410128 [2]湖南省林业科学院,湖南长沙410018

出　　处：《湖南农业科学》2024年第9期73-77,共5页Hunan Agricultural Sciences

基　　金：湖南省林业科技创新与攻关项目(科学研究)(XLKY202211);湖南省林业草原防灾减灾、生态保护与修复项目(科学研究)(SB2023A004);2024年中央财政推广项目(〔2024〕XT13号)。

摘　　要：为获得具有杀松材线虫活性的菌株,在湖南省浏阳市采集了健康马尾松、感染松材线虫病但尚未死亡的马尾松以及感染松材线虫病致死的马尾松材料,从中分离得到了27株内生细菌进行杀线活性的测定,并对高效拮抗菌株进行了鉴定。结果表明:从感染但尚未死亡的松树分离得到的细菌H-4都具有较高的杀线活性;在发酵液处理48 h后,菌株H-4的杀松材线虫活性最高,松材线虫校正死亡率和消解率分别达到97.1%和89.2%。综合考虑菌株H-4的形态学特征、生理生化测定结果以及16S rRNA系统发育树分析结果,确定菌株H-4为暹罗芽孢杆菌。To obtain the strains capable of killing pine wood nematodes,we collected the healthy plants,the plants infected with pine wood nematodes but still alive,and the plants infected with pine wood nematodes and dead of Pinus massoniana from Liuyang City,Hunan Province.A total of 27 strains of endophytic bacteria were isolated and subjected to assessment of their nematocidal effects.Furthermore,the efficient antagonistic strains were identified.The results showed that strain H-4 isolated from the infected but still alive plants exhibited a high nematocidal activity.The treatment with the fermentation broth of strain H-4 for 48 h achieved the highest killing effect on pine wood nematodes,with the corrected mortality and clearance rates of pine wood nematodes reaching 97.1%and 89.2%,respectively.Strain H-4 was identified as Bacillus siamensis according to the morphological,physiological,and biochemical characteristics and the phylogenetic tree built based on the 16S rRNA sequence.

关 键 词：松材线虫 内生细菌 暹罗芽孢杆菌 

分 类 号：S763[农业科学—森林保护学]