烟草青枯菌裂解性噬菌体ØPB2的稳定性分析及应用  

Stability and Application of Lytic BacteriophageϕPB2 against Ralstonia solanacearum

作　　者：蔡刘体 陆宁 代园凤 杨颜 唐军 陈兴江 孟建玉 汪汉成 CAI Liuti;LU Ning;DAI Yuanfeng;YANG Yan;TANG Jun;CHEN Xingjiang;Meng Jianyu;WANG Hancheng(Guizhou Academy of Tobacco Science,Guiyang 550081,China;Guizhou Provincial Company of China National Tobacco Corporation,Guiyang 550000,China;Qiandongnan Company of Guizhou Provincial Tobacco Company,Kaili 556099,China)

机构地区：[1]贵州省烟草科学研究院,贵阳550081 [2]中国烟草总公司贵州省公司,贵阳550000 [3]贵州省烟草公司黔东南州分公司,凯里556099

出　　处：《中国生物工程杂志》2024年第8期78-84,共7页China Biotechnology

基　　金：中国烟草总公司项目(110202101048(LS-08));中国烟草总公司贵州省公司项目(2020XM03)资助项目。

摘　　要：为掌握烟草青枯菌噬菌体ØPB2的稳定性及其对烟草青枯病的防控效果,测定了温度、pH、盐浓度对其活性的影响,采用离体叶片实验检测了噬菌体对烟草叶片青枯病病斑的防控效果,并通过大田试验检测了田间防控效果。结果表明,噬菌体ØPB2在低温和室温水体条件下,30~90 d内比较稳定;在pH为5.0~8.0较稳定;离体叶片接种不同浓度噬菌体液,噬菌体浓度越高,防控效果越好;噬菌体ØPB2菌剂田间防控青枯病,青枯病发病率和病情指数较空白对照明显降低,噬菌体菌剂处理平均相对防控率达到70%以上(2021年)。研究结果为烟草青枯病生物防控提供一种新策略,为利用烟草青枯菌裂解性噬菌体防控烟草青枯病提供理论依据。In order to study the stability of the lytic bacteriophageϕPB2 and its effect against Ralstonia solanacearum,the effects of temperature,pH value,and salt concentration on its activity were measured;the control effect ofϕPB2 on tobacco bacterial wilt was tested by in vitro leaf experiments,and the control effect was detected by field experiments.The results showed that bacteriophageϕPB2 was relatively stable in water at low temperature and room temperature for 1-3 months,and in the pH range of 5.0-8.0.The bacterial wilt disease spots on tobacco leaves after inoculation of different concentrations of theϕPB2 showed better prevention and control effects with higher concentration ofϕPB2 compared to the control;the higher concentration ofϕPB2,the better effect of prevention and control.Compared with CK,the bacterial wilt rate and disease index of the treatment by theϕPB2 were significantly lower,and the average relative control rate reached more than 70%(in 2021).These results may provide a basis for the biological control strategies of bacterial wilt of flue-cured tobacco,and provide a theoretical basis for the use of lytic bacteriophage to control bacterial wilt of tobacco.

关 键 词：烟草 烟草青枯病 青枯菌噬菌体 

分 类 号：Q939.48[生物学—微生物学] S435.72[农业科学—农业昆虫与害虫防治]