AlphaFold-guided redesign of a plant pectin methylesterase inhibitor for broad-spectrum disease resistance  

作　　者：Yeqiang Xia Guangzheng Sun Junhua Xiao Xinyi He Haibin Jiang Zhichao Zhang Qi Zhang Kainan Li Sicong Zhang Xuechao Shi Zhaoyun Wang Lin Liu Yao Zhao Yuheng Yang Kaixuan Duan Wenwu Ye Yiming Wang Suomeng Dong Yan Wang Zhenchuan Ma Yuanchao Wang 

机构地区：[1]Department of Plant Pathology,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China [2]Key Laboratory of Soybean Disease and Pest Control(Ministry of Agriculture and Rural Affairs),Nanjng Agricultural University,Nanjng,Jiangsu 210095,China [3]Anhui Province Key Laboratory of Crop Integrated Pest Management,Anhui Agricultural University,Hefei 230036,China [4]School of Bioengineering,Dalian University of Technology,Dalian 116024,China [5]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Bejing 100193,China

出　　处：《Molecular Plant》2024年第9期1344-1368,共25页分子植物（英文版）

基　　金：supported bythe National Key Research and Development Program of China(2022YFF1001500);the National Natural Science Foundation of China(32102172)and(31721004);the China National Postdoctoral Program for Innovative Talents(BX2021130);the China Postdoctoral Science Foundation(2021M700074).

摘　　要：Plant cell walls are a critical site where plants and pathogens continuously struggle for physiological domi-nance.Here we show that dynamic remodeling of pectin methylesterification of plant cell walls is a compo-nent of the physiological and co-evolutionary struggles between hosts and pathogens.A pectin methyles-terase(PsPME1)secreted by Phytophthora sojae decreases the degree of pectin methylesterification,thus synergizing with an endo-polygalacturonase(PsPG1)to weaken plant cell walls.To counter PsPME1-mediated susceptibility,a plant-derived pectin methylesterase inhibitor protein,GmPMl1,protects pectin to maintain a high methylesterification status.GmPMl1 protects plant cell walls from enzymatic degrada-tion by inhibiting both soybean and P.sojae pectin methylesterases during infection.However,constitutive expression of GmPMl1 disrupted the trade-off between host growth and defense responses.We therefore used AlphaFold structure tools to design a modified form of GmPMI1(GmPMI1R)that specifically targets and inhibits pectin methylesterases secreted from pathogens but notfrom plants.Transient expression of GmPMi1R enhanced plant resistance to oomycete and fungal pathogens.In summary,our work highlights the biochemical modification of the cell wall as an important focal point in the physiological and co-evolutionary conflict between hosts and microbes,providing an important proof of concept that Al-driven structure-based tools can accelerate the development of new strategies for plant protection.

关 键 词：apoplastic immunity AlphaFold-guided redesign broad-spectrum disease resistance pectin methyl-esteraseinhibitor cell wall 

分 类 号：Q94[生物学—植物学]