人参皂苷Rg3减轻帕金森病小鼠神经系统氧化应激损伤的作用机制  

The Mechanism of Action of Ginsenoside Rg3 for the Relief from Oxidative Stress Injury in the Nervous System of Mice with Parkinson′s Disease

作　　者：杨倩 王灿 YANG Qian;WANG Can(Department of Neurology,Hunan Brain Hospital,Changsha 410002,China)

机构地区：[1]湖南省脑科医院神经内科,湖南长沙410002

出　　处：《西部中医药》2024年第9期25-29,共5页Western Journal of Traditional Chinese Medicine

基　　金：湖南省脑科医院科研基金课题(20201642)。

摘　　要：目的:探究人参皂苷Rg3减轻帕金森病(Parkinson′s disease,PD)小鼠神经系统氧化应激损伤的作用机制。方法:将60只C57BL/6小鼠分为对照组、PD组、低剂量干预组和高剂量干预组,每组15只。通过腹腔注射1-甲基-4-苯基-1,2,3,6-四氢吡啶(1-methy1-4-phenvl-1,2,3,6-tetrahvdropvridine,MPTP)构建PD小鼠模型,建模前3天,低、高剂量干预组小鼠分别腹腔注射3、12 mg/kg人参皂苷Rg3,造模后继续干预28天,对照组和PD组腹腔注射等量生理盐水。各组小鼠腹腔注射第4、8、12天时进行爬杆实验和旋转实验评估运动能力;末次腹腔注射完成后,断颈处死小鼠取脑组织制成冰冻切片,免疫组化分析黑质纹状体中酪氨酸羟化酶(tyrosine hydroxylase,TH)表达水平;酶联免疫吸附法测定黑质纹状体中超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)水平;Western blot方法检测黑质纹状体中TH、核因子E2相关因子2(nuclear factor erythroid 2-related factor 2,Nrf2)、血红素氧合酶1(heme oxygenase-1,HO-1)、醌氧化还原酶1(quinoneoxidoreductase 1,NQO1)蛋白表达水平。结果:与对照组比较,PD组小鼠爬杆时间延长、旋转停留时间缩短、MDA水平升高,TH阳性率、SOD、GSH-PX活性、TH、Nrf2、HO-1、NQO1蛋白水平均降低,差异有统计学意义(P<0.05);与PD组比较,低、高剂量干预组小鼠爬杆时间缩短,旋转停留时间延长,MDA水平降低,TH阳性率、SOD、GSH-PX活性、TH、Nrf2、HO-1、NQO1蛋白水平均升高,差异有统计学意义(P<0.05)。结论:人参皂苷Rg3可能通过激活黑质纹状体中Nrf2/HO-1/NQO1通路,减轻PD小鼠神经系统氧化应激损伤,而改善其病理状态。Objective:To survey the mechanism of ginsenoside Rg3 alleviating oxidative stress injury in the nervous system of mice with Parkinson's disease(PD).Methods:Sixty C57BL/6 mice were divided into the control group,PD group,low-dose intervention group and high-dose intervention group with 15 ones in each group.PD mice models were constructed using intraperitoneal injection of MPTP,the mice in the low-dose intervention group and high-dose intervention group were intraperitoneally injected with 3 and 12 mg/kg ginsenoside Rg3 respectively three days before the modeling,and intervened for 28 days after the modeling,the control group and PD group accepted abdominal injection of an equal amount of physiological saline.Motor ability was assessed by pole-climbing test and rotation experiment on the forth,eighth and 12th day of intraperitoneal injection in each group of mice;after completing the last intraperitoneal injection,mice were sacrificed by cervical dislocation and brain tissues were taken to make frozen sections,immunohistochemical analysis was carried out to detect the expressions of tyrosine hydroxylase(TH)in substantia nigra striata;ELISA was applied to measure the levels of SOD,MDA and GSH-PX;Western blot was used to measure the expressions of TH,Nrf-2,HO-1 and NQ01 protein in substantia nigra striata.Results:Compared with the control group,poleclimbing time,rotation times,rotation time and the levels of MDA increased in the mice of PD group,while TH positive rate,the activity of SOD and GSH-PX,the levels of TH,Nrf2,HO-1 and NQ01 protein decreased,and the difference had statistical meaning(P<0.05);compared with PD group,pole-climbing time,rotation times,rotation time and the levels of MDA reduced in low and high dose intervention groups while TH positive rate,the activity of SOD and GSH-PX,the levels of TH,Nrf2,HO-1 and NQ01 protein increased,and the difference was statistically significant(P<0.05).Conclusion:Ginsenoside Rg3 could improve its pathological state possibly through activating Nrf2/HO-1/NQ01 pathway

关 键 词：帕金森病 黑质纹状体 氧化应激损伤 人参皂苷RG3 小鼠 动物实验 

分 类 号：R277.7[医药卫生—中医学]