恒清Ⅴ号方干预海人藻酸致癫痫小鼠的作用机制研究  

Effects of HengqingⅤPrescription on Epilepsy Mice Induced by Kainic Acid

作　　者：邓楚珺 竺闻雷 唐剑飞[1] 沈洁[1] 陈慧泽 袁菁 孟胜喜[1] DENG Chujun;ZHU Wenlei;TANG Jianfei;SHEN Jie;CHEN Huize;YUAN Jing;MENG Shengxi(Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 200233,China)

机构地区：[1]上海交通大学医学院附属第六人民医院,上海200233

出　　处：《中西医结合心脑血管病杂志》2024年第19期3529-3533,共5页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：上海市第六人民医院“脑科学与类脑研究”脑项目功能和针灸脑图谱光声成像技术应用研究(No.ynnkxyb202415);上海市第六人民医院医疗服务能级提升工程临床医疗技术骨干队伍培育项目(No.20220213);上海市第六人民医院院级课题(No.ynxg202218);上海交通大学医学院“大学生创新训练计划”项目(No.1723Z002,1824021);上海市进一步加快中医药传承创新发展三年行动计划项目(2021—2023年)[No.ZY(2021-2023)-0205-04];华东片区及市级中医专科专病联盟建设项目[No.ZY(2021-2023)-0302]。

摘　　要：目的:探讨恒清Ⅴ号方干预海人藻酸致癫痫小鼠的作用机制。方法:选取雄性野生型C57BL/6J小鼠40只,随机分为正常对照组、模型组、恒清Ⅴ号方干预组及丙戊酸钠干预组,每组10只。恒清Ⅴ号方干预组给予恒清Ⅴ号方灌胃,丙戊酸钠干预组给予丙戊酸钠溶液腹腔注射,正常对照组及模型组给予生理盐水灌胃,每日1次,持续10 d。最后1次给药干预后,模型组、恒清Ⅴ号方干预组及丙戊酸钠干预组均给予海人藻酸腹腔注射以诱发小鼠癫痫发作,观察各组小鼠行为变化及空间工作记忆能力。观察结束后检测各组小鼠海马组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和总抗氧化能力(T-AOC)水平。结果:与正常对照组比较,模型组小鼠癫痫持续状态时间明显延长,癫痫潜伏期缩短,Ⅲ级以上惊厥发作次数增加,新异臂探索次数、新异臂探索时间及新异臂探索路程均减少,差异均有统计学意义(P<0.01)。与模型组比较,恒清Ⅴ号方干预组与丙戊酸钠干预组癫痫持续状态时间均明显缩短,癫痫潜伏期延长,Ⅲ级以上惊厥发作次数减少,新异臂探索次数、新异臂探索时间及新异臂探索路程均增加,差异均有统计学意义(P<0.01)。与丙戊酸钠干预组比较,恒清Ⅴ号方干预组癫痫持续状态时间缩短,癫痫潜伏期时间延长,Ⅲ级以上惊厥发作次数减少,新异臂探索次数、新异臂探索时间及新异臂探索路程均增加,差异均有统计学意义(P<0.05)。与正常对照组比较,模型组小鼠海马组织中MDA水平升高(P<0.01),SOD、CAT及T-AOC水平下降(P<0.01)。与模型组比较,恒清Ⅴ号方干预组、丙戊酸钠干预组小鼠海马组织中MDA水平均下降(P<0.01),SOD、CAT及T-AOC水平均升高(P<0.01)。与丙戊酸钠干预组比较,恒清Ⅴ号方干预组小鼠海马组织中MDA水平均下降(P<0.05),SOD、CAT及T-AOC水平均升高(P<0.05)。结论:恒清Ⅴ号方可以干预海�Objective:To explore effects of HengqingⅤPrescription on epilepsy mice induced by kainic acid.Methods:Forty male wild-type C57BL/6J mice were randomly divided into normal control group,model group,HengqingⅤPrescription intervention group,and valproate intervention group,with 10 mice in each group.The mice in intervention group of HengqingⅤPrescription were administrated with HengqingⅤPrescription by gavage.The mice in valproate intervention group were administrated with valproate by intraperitoneal injection,and the mice in normal control group and model group were administrated with physiological saline by gavage,once a day for 10 days.After the last administration,except for normal control group,the other groups were injected intraperitoneal with kainic acid to induce seizures.The behavioral changes and spatial working memory ability were observed.After the observation,the levels of malondialdehyde(MDA),superoxide dismutase(SOD),catalase(CAT),and total antioxidant capacity(T-AOC)in the hippocampus of mice in each group were measured.Results:Compared with normal control group,model group mice showed significantly longer duration of epileptic state,shorter latency of epilepsy,higher frequency of seizures above grade 3,and less number of new arm explorations,time of new arm explorations,and exploration distance of new arm explorations(all P<0.01).Compared with model group,the duration of epileptic state in Hengqing V Prescription intervention group and the intervention group of valproate significantly shortened,the latency time of epilepsy prolonged,the number of seizures above grade 3 reduced,number of new arm explorations,time of new arm explorations,and exploration distance of new arm explorations all increased(all P<0.01).Compared with valproate intervention group,mice in the HengqingⅤPrescription intervention group showed a shorter duration of epileptic state,a longer latency of epilepsy,fewer seizures above grade 3,and an increase in the number,time,distance of explorations in new arm explorations(

关 键 词：癫痫 恒清Ⅴ号方 海人藻酸 氧化应激 超氧化物歧化酶 实验研究 

分 类 号：R285.5[医药卫生—中药学]