三白纳米口服液制备工艺优化及抗氧化活性研究  

Preparation optimization and antioxidation activity of Sanbai nano oral liquid

作　　者：陶稳稳 肖垚垚 刘心雨 赵亚男 黄顺旺 张彩云 TAO Wen-wen;XIAO Yao-yao;LIU Xin-yu;ZHAO Ya-nan;HUANG Shun-wang;ZHANG Cai-yun(Anhui Key Laboratory of Pharmaceutical Preparation Technology and Application,School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012;Engineering Technology Research Center of Modern Pharmaceutical Preparation,Education Bureau of Anhui,Hefei 230012;Anhui Genuine Chinese Medicinal Materials Quality Improvement Innovation Collaborative Center,Hefei 230012;Institute of Pharmaceutics,Anhui Academy of Chinese Medicine,Hefei 230012;Anhui Key Laboratory of Compound Chinese Materia Medica,Hefei 230012;Hefei Cancer Hospital,Chinese Academy of Sciences,Hefei 231000;Hefei Innovative Medical Technology Co.,Ltd.,Hefei 231000)

机构地区：[1]安徽中医药大学药学院,药物制剂技术与应用安徽省重点试验室,合肥230012 [2]安徽省教育厅现代药物制剂工程技术研究中心,合肥230012 [3]安徽省道地中药材品质提升创新协同中心,合肥230012 [4]安徽省中医药科学院药物制剂研究所,合肥230012 [5]中药复方安徽省重点试验室,合肥230012 [6]中国科学院合肥肿瘤医院,合肥231000 [7]合肥创新医药技术有限公司,合肥231000

出　　处：《中南药学》2024年第9期2320-2325,共6页Central South Pharmacy

基　　金：安徽省科技重大专项项目(No.202203a07020031);安徽省教育厅基金项目(No.KJ2021ZD0065);大学生创新创业训练计划项目(No.202110369021)。

摘　　要：目的优化中药复方三白汤的提取工艺和醇沉工艺,制备均匀、稳定、有效的三白纳米口服液。方法通过单因素考察和正交试验优化三白汤水煎煮提取和醇沉工艺,以芍药苷和甘草酸含量作为指标确定三白纳米口服液制备参数。考察三白纳米口服液的外观、粒径、Zeta电位和粒子形态,进行感官评价和含糖量测试,考察其对2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)阳离子自由基的消除能力。结果三白纳米口服液的最佳制备工艺为药材加12倍水浸泡1 h,煎煮1.5 h,煎煮3次,合并提取液用75%乙醇进行沉淀,浓缩、离心处理后,加入0.4 g·kg^(-1)山梨酸钾和0.1 g·kg^(-1)甜菊糖苷即可。所得口服液中含有平均粒径133.5 nm、PDI值0.108、Zeta电位为-17.7 mV的类球形纳米粒子,口服液的含糖量为3.1 g·L^(-1),其对ABTS阳离子自由基消除IC50为1.235 mg·mL^(-1)。结论本研究成功制备了三白纳米口服液,其美白活性成分芍药苷和甘草酸含量高,具有良好自由基消除活性,为经典美白复方三白汤新产品的开发提供了技术参数和科学试验依据。Objective To optimize the extraction and sedimentation of Sanbai decoction,and to prepare uniform,stable and effective Sanbai nano oral liquid.Methods The extraction and alcohol precipitation process of Sanbai decoction were optimized by single factor test and orthogonal experiment.The preparation parameters of Sanbai nano oral liquid were determined by the content of paeoniflorin and glycyrrhizic acid.The appearance,particle size,Zeta potential,and morphology of the oral liquid were evaluated.Sensory evaluation and sugar content were tested.Finally,its free radical scavenging ability was investigated by eliminating 2,2'-diazo-di-3-ethylbenzothiazolin-6-sulfonic acid(ABTS)cationic radicals.Results The best preparation of Sanbai nano oral liquid was as follows:soaking the herbs in 12 times of water for 1 h,decoction 3 times and 1.5 h each time.The combined extract was precipitated with 75%ethanol.After the concentration and centrifugation,0.4-1-1 g·kg potassium sorbate and 0.1 g·kg stevia glycoside were added.The obtained oral liquid contained spheroid nanoparticles with an average particle size of 133.5 nm,the PDI value of 0.108-and Zeta potential of-17.7 mV.The sugar content of the oral liquid was 3.1 g·L^(-1),and the IC50-of ABTS cationic free radical elimination was 1.235 mg·mL 1.Conclusion Sanbai nano oral liquid is successfully prepared with high content of whitening active ingredients paeoniflorin and glycyrrhizic acid,which has good free radical scavenging ability,and provides technical parameters and experimental basis for the development of new products.

关 键 词：三白汤 三白纳米口服液 正交试验 芍药苷 甘草酸 抗氧化活性 

分 类 号：R283.6[医药卫生—中药学] R285[医药卫生—中医学]