响应面法优化涡旋辅助提取胆木中异长春花苷内酰胺的工艺  

Optimization of vortex-assisted extraction of strictosamide from Nauclea officinalis by response surface method

作　　者：樊小红 吕菲菲[1] 赵祥升[1] 魏建和[1,2] FAN Xiao-hong;LYU Fei-fei;ZHAO Xiang-sheng;WEI Jian-he(Key Laboratory of Resources Conservation and Development of Southern Medicine of Hainan&Hainan Branch of Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Haikou 570311;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education&National Engineering Laboratory for Breeding of Endangered Medicinal Materials,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193)

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所海南分所,海南省南药资源保护与开发重点实验室,海口570311 [2]中国医学科学院北京协和医学院药用植物研究所,中草药物质基础与资源利用教育部重点实验室,濒危药材繁育国家工程实验室,北京100193

出　　处：《中南药学》2024年第9期2350-2354,共5页Central South Pharmacy

基　　金：海南省自然科学基金青年基金项目(No.821QN0948);中国医学科学院医学与健康科技创新工程创新项目(No.2021-I2M-1-032)。

摘　　要：目的采用响应面法优化胆木中异长春花苷内酰胺的涡旋辅助提取工艺。方法在单因素试验的基础上通过Box-Behnken方法对乙醇浓度、涡旋转速、液料比等参数进行优化,建立参数与响应值之间的数学模型,确定最佳提取工艺。结果生物碱的最佳工艺为:乙醇浓度55%,涡旋转速1500 r·min^(-1),液料比60 mL/g,涡旋时间1 min,该条件下异长春花苷内酰胺的实际提取率为3.85 mg·g^(-1),与理论模拟值(3.82 mg·g^(-1))接近。结论该提取方法简便、快速、提取率高,适用于胆木生物碱的提取,研究结果为胆木的产业化加工提供了理论基础。Objective To optimize the vortex-assisted extraction of strictosamide from Nauclea officinalis with the response surface method.Methods The extraction parameters such as ethanol concentration,vortex rotation,and liquid-solid ratio were optimized through Box-Behnken design based on single-factor experiments.The mathematical model between the parameters and the response values was established to determine the best vortex extraction.Results The optimal conditions included ethanol concentration at 55%,vortex rotation at 1500 r·min^(-1),liquid-solid ratio of 60 mL/g,and vortex time for 1 min.Under these conditions,the yield of strictosamide was 3.85 mg·g^(-1),close to the theoretical value of 3.82 mg·g^(-1).Conclusion The extraction is simple,fast and efficient,which is suitable for extracting alkaloids from Nauclea officinalis.It provides a theoretical basis for the industrial process of Nauclea officinalis.

关 键 词：胆木 异长春花苷内酰胺 涡旋 响应面分析 提取工艺 

分 类 号：R284.1[医药卫生—中药学]