金露梅不同药用部位6种黄酮类成分的含量比较  

Content of 6 flavonoids in medicinal parts of Dasiphora fruticosa(L.)Rydb

作　　者：李永生[1] 耿飞飞 陈志永 任慧[3] 胡静 鲁文静[3] 崔小敏[3] LI Yong-sheng;GENG Fei-fei;CHEN Zhi-yong;REN Hui;HU Jing;LU Wen-jing;CUI Xiao-min(Honghui Hospital,Xi’an Jiaotong University of Medicine School,Xi’an 710054;Shaanxi University of Chinese Medicine,Xianyang Shaanxi 712046;Shaanxi Academy of Traditional Chinese Medicine,Xi’an 710016)

机构地区：[1]西安交通大学医学院附属红会医院,西安710054 [2]陕西中医药大学,陕西咸阳712046 [3]陕西省中医药研究院,西安710016

出　　处：《中南药学》2024年第9期2433-2437,共5页Central South Pharmacy

基　　金：西安市科技局医学研究项目(No.23YXYJ0033);陕西省重点研发计划一般项目(No.2022SF-577,No.2021SF-279);北京康盟慈善基金会医学科研发展基金项目(No.B21114FN);陕西省中医药研究院“苗圃培育计划”项目(No.2021-05)。

摘　　要：目的建立同时测定金露梅叶、花、果和枝4个不同药用部位黄酮类成分儿茶素、槲皮素-3-O-(6''-没食子酰基)-β-D-半乳糖苷、金丝桃苷、异槲皮苷、萹蓄苷和银锻苷含量的方法,并对不同部位含量结果进行比较。方法采用Agilent Poroshell 120 PFP色谱柱(4.6 mm×150 mm,2.7μm),以乙腈-0.1%磷酸水溶液为流动相进行梯度洗脱,切换检测波长分别为280 nm和354 nm,柱温为30℃,流速为0.8 mL·min^(-1)。采用柱状图及堆积柱状图表征不同药用部位6种化学成分的含量差异,Chiplot科研绘图平台对不同化学成分含量进行Spearman相关性分析。结果6种化学成分的总含量在不同部位的分布为:花>叶>果>枝,Spearman相关性分析结果表明,金露梅中异槲皮苷和金丝桃苷、异槲皮苷和银锻苷、金丝桃苷和银锻苷的含量具有较强的正相关性,相关系数分别为0.98、0.93和0.93。结论该研究结果可为金露梅质量控制、临床应用和资源的合理开发利用提供依据。Objective To establish a method to determine the content of catechin,quercetin-3-O-(6''-galloyl)-β-D-galactopyranoside,hyperoside,isoquercitrin,avicularin and tiliroside in 4 medicinal parts of Dasiphora fruticosa(L.)Rydb,including leaves,flowers,fruits and branches,and to compare the content in different medicinal parts.Methods Agilent Poroshell 120 PFP column(4.6 mm×150 mm,2.7μm)was used.Acetonitrile-0.1%phosphoric acid aqueous solution was used as the mobile phase for gradient elution,with the switching detection wavelength of 280 nm and 354 nm.The-column temperature was 30℃,and the flow rate was 0.8 mL·min 1.The histogram and stacked column chart were plotted to characterize the content of 6 chemical components in different medicinal parts.The Spearman correlation analysis of the content of chemical components was conducted with“Chiplot”mapping platform.Results The total content of the 6 chemical components in different parts was as follows:flowers>leaves>fruits>branches.Spearman correlation analysis showed a strong positive correlation between the content of isoquercitrin and hyperoside,isoquercitrin and tiliroside,hyperoside and tiliroside,with correlation coefficients of 0.98,0.93 and 0.93,respectively.Conclusion The study provides a basis for the quality control,clinical application,and rational development and utilization of Dasiphora fruticosa.

关 键 词：金露梅 不同药用部位 黄酮 含量比较 

分 类 号：R284.1[医药卫生—中药学]